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Induction of system A amino acid transport activity through long-term treatment with ouabain: possible correlation with enhanced (Na/sup +//K/sup +/)ATPase activity

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6173455
; ; ;

Mouse embryo fibroblast cells (C3H-10T1/2) and the methylcholanthrene-transformed derivative (MCA-10T1/2) were treated with basal modified Eagles medium at varying ouabain concentrations ranging from 0.05 mM to 0.5 mM for 16 h in culture. After replacing the ouabain-containing medium with BME, System A (/sup 3/H-AlB uptake) and the (Na/sup +//K/sup +/)ATPase pump activity (ouabain-sensitive /sup 86/Rb/sup +/ uptake) was increased 10-fold and 3-fold, respectively (at 0.4 mM ouabain) in confluent C3H-10T1/2 cells. System A and the (Na/sup +//K/sup +/)ATPase activity was increased 15-fold and 5-fold, respectively in confluent MCA-10T1/2 cells but the increase was maximal at 0.2 mM ouabain. This treatment with ouabain increased the (Na/sup +/)/sub i//(K/sup +/)/sub i/ as measured by atomic absorption, and thereby decreased the Na/sup +/ and K/sup +/ electrochemical gradients. Their data show that the transformed cells were more sensitive to the internal ion inversion by ouabain than the C3H-10T1/2 cells. It appears, from data on hypertonicity and lipophilic cations that neither the chemical Na/sup +/ gradient nor the negative membrane potential are the primary driving forces of System A transport.

Research Organization:
Cornell Univ., Ithaca, NY
OSTI ID:
6173455
Report Number(s):
CONF-870644-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 46:6; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

3-METHYLCHOLANTHRENE
550201* -- Biochemistry-- Tracer Techniques
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ABSORPTION SPECTROSCOPY
ACID ANHYDRASES
ALKALI METAL ISOTOPES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AROMATICS
ATP-ASE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CARBOHYDRATES
CARBOXYLIC ACIDS
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DRUGS
EMBRYONIC CELLS
ENZYME ACTIVITY
ENZYMES
FIBROBLASTS
GLYCOSIDES
HYDROCARBONS
HYDROLASES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MEMBRANE TRANSPORT
MICE
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
OUABAIN
PHOSPHOHYDROLASES
POLYCYCLIC AROMATIC HYDROCARBONS
RADIOISOTOPES
RODENTS
RUBIDIUM 86
RUBIDIUM ISOTOPES
SOMATIC CELLS
SPECTROSCOPY
STROPHANTHINS
TOXICITY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES