Mechanisms of ouabain resistance
Thesis/Dissertation
·
OSTI ID:6151843
Experiments were designed to investigate the mechanism of ouabain resistance in two distinct types of transfected cells derived from ouabain-sensitive CV-1 cell parents. The first type of transfectant is the recipient of a gene encoding the alpha subunit of the rodent renal Na,K-ATPase (R-alphal gene); the second type of transfectant is the recipient of the mouse ouabain resistance gene. Measurements of /sup 86/Rb/sup +/ uptake and Na,K=ATPase activity in R-alphal gene transfectant cells and CV-1 parent cells indicate that the ouabain-resistant phenotype of the transfectants is due to expression of a relatively ouabain-insensitive Na,K=ATPase. CV-1 parent cells express one component of ouabain sensitive /sup 86/Rb/sup +/ uptake and one component of ouabain-sensitive Na, K-ATPase activity. R-alpha 1 gene transfectants express the parental forms of ouabain-sensitive /sup 86/Rb/sup +/ uptake and Na,K-ATPase activity, but in addition express new,relatively ouabain-insensitive forms of /sup 86/Rb/sup +/ uptake activity and Na,K-ATPase activity.
- Research Organization:
- Harvard Univ., Boston, MA (USA)
- OSTI ID:
- 6151843
- Country of Publication:
- United States
- Language:
- English
Similar Records
Structure-function relationships in the Na,K-ATPase. cap alpha. subunit: site-directed mutagenesis of glutamine-111 to arginine and asparagine-122 to aspartic acid generates a ouabain-resistant enzyme
Altered sensitivity of system A amino acid transport to ouabain in normal and transformed C3H-10T1/2 cells during the cell cycle
Ouabain-sensitive Rb+ uptake in mouse eggs and preimplantation conceptuses
Journal Article
·
Mon Oct 31 23:00:00 EST 1988
· Biochemistry; (United States)
·
OSTI ID:6143653
Altered sensitivity of system A amino acid transport to ouabain in normal and transformed C3H-10T1/2 cells during the cell cycle
Journal Article
·
Tue Jan 31 23:00:00 EST 1989
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
·
OSTI ID:5037391
Ouabain-sensitive Rb+ uptake in mouse eggs and preimplantation conceptuses
Journal Article
·
Mon Jul 01 00:00:00 EDT 1991
· Developmental Biology; (United States)
·
OSTI ID:5464127
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ALKALI METAL ISOTOPES
ANIMAL CELLS
ANIMALS
ATP-ASE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYME ACTIVITY
ENZYMES
GENE REGULATION
GENES
GLYCOSIDES
HYDROLASES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
MAMMALS
MICE
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
OUABAIN
PHENOTYPE
PHOSPHOHYDROLASES
RADIOISOTOPES
RODENTS
RUBIDIUM 86
RUBIDIUM ISOTOPES
STROPHANTHINS
TOLERANCE
TRACER TECHNIQUES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ALKALI METAL ISOTOPES
ANIMAL CELLS
ANIMALS
ATP-ASE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYME ACTIVITY
ENZYMES
GENE REGULATION
GENES
GLYCOSIDES
HYDROLASES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
MAMMALS
MICE
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
OUABAIN
PHENOTYPE
PHOSPHOHYDROLASES
RADIOISOTOPES
RODENTS
RUBIDIUM 86
RUBIDIUM ISOTOPES
STROPHANTHINS
TOLERANCE
TRACER TECHNIQUES
VERTEBRATES