Identification of a celE-binding protein and its potential role in induction of the celE gene in Thermomonospora fusca

Journal Article · Thu Sep 01 04:00:00 EDT 1988 · Journal of Bacteriology; (USA)

OSTI ID:7019013

Lin, E; Wilson, D B ^[1]

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1. Cornell Univ., Ithaca, NY (USA)

Thermomonospora fusca cellulase E{sub 5} is encoded by the celE gene. This appears to be regulated at the transcriptional level by both induction and repression, and three putative closely linked promoters have been located by S1 mapping. To study its regulatory mechanism, a gel retardation assay was used to identify a protein in T. fusca cell extracts that interacted specifically with the DNA fragment containing the celE promoters. It was found that the binding activity appeared only when cellulase synthesis was induced, and it therefore resembled an activator protein involved in cellulase induction. DNase I footprinting identified the target sequence for this protein as a 21-base-pair sequence downstream from the putative celE promoters. The level of this protein was measured in two cellulase constitutive mutants, and the results suggest a complex control for celE induction.

• DOE Contract Number: FG02-84ER13233
• OSTI ID: 7019013
• Journal Information: Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 170:9; ISSN JOBAA; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
BACTERIA
BIOLOGICAL FUNCTIONS
CELLULASE
DNA-ASE
ENZYME INDUCTION
ENZYMES
ESTERASES
FUNCTIONS
GENE REGULATION
GENE REPRESSORS
GENES
GLYCOSYL HYDROLASES
HYDROLASES
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEOPROTEINS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PROTEINS