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Thermostable cellulase from a thermomonospora gene

Patent ·
OSTI ID:871182
 [1];  [1];  [1]
  1. Ithaca, NY
+ Show Author Affiliations

The invention relates to a gene isolated from Thermomonospora fusca, wherein the gene encodes a thermostable cellulase. Disclosed is the nucleotide sequence of the T. fusca gene; and nucleic acid molecules comprising the gene, or a fragment of the gene, that can be used to recombinantly express the cellulase or a catalytically active polypeptide thereof, respectively. The isolated and purified recombinant cellulase or catalytically active polypeptide may be used to hydrolyze substrate either by itself; or in combination with other cellulases, with the resultant combination having unexpected hydrolytic activity.

Research Organization:
Cornell University
DOE Contract Number:
FG02-84ER13233
Assignee:
Cornell Research Foundation, Inc. (Ithaca, NY)
Patent Number(s):
US 5677151
Application Number:
08/265429
OSTI ID:
871182
Country of Publication:
United States
Language:
English

References (6)

Biochemistry and Genetics of Actinomycete Cellulases journal January 1992
Hydrolysis of Cellulose by Saturating and Non–Saturating Concentrations of Cellulase: Implications for Synergism journal March 1988
Engineering cellulase mixtures by varying the mole fraction of Thermomonospora fusca E5 and E3, Trichoderma reesei CBHI, and Caldocellum saccharolyticum β-glucosidase journal November 1993
Synergism between pure Thermomonospora fusca and Trichoderma reesei cellulases journal January 1993
Activity studies of eight purified cellulases: Specificity, synergism, and binding domain effects journal October 1993
Fragmentation of cellulose by the majorThermomonospora fusca cellulases,Trichoderma reesei CBHI, and their mixtures journal November 1992

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Related Subjects

/435/424/999/
acid
acid molecule
acid molecules
active
active poly
active polypeptide
activity
catalytically
catalytically active
cellulase
cellulases
combination
comprising
disclosed
encodes
express
fragment
fusca
hydrolytic
hydrolyze
isolated
molecules
molecules comprising
nucleic
nucleic acid
nucleotide
nucleotide sequence
polypeptide
purified
recombinant
recombinantly
relates
respectively
resultant
sequence
substrate
thermomonospora
thermostable
thermostable cellulase
unexpected