Transcription of the celE gene in Thermomonospora fusca

Journal Article · Thu Sep 01 04:00:00 EDT 1988 · Journal of Bacteriology; (USA)

OSTI ID:7165367

Lin, E ^[1]; Wilson, D B ^[2]

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1. State Univ. of New York, Brooklyn (USA)
2. Cornell Univ., Ithaca (USA)

The steady-state level of celE mRNA (coding for cellulase E{sub 5}) in Thermomonospora fusca YX was measured by Northern (RNA blot) hybridization under conditions causing induction or repression of cellulase synthesis. A good correlation was found between the mRNA level of cellulase E{sub 5}, suggesting that the T. fusca celE gene is regulated at the level of mRNA and, most likely, at the level of transcription. The 5{prime} and 3{prime} ends of the celE gene transcription unit were determined by S1 mapping with single-stranded DNA probes. These results showed that there were three species of celE mRNA in T. fusa YX with closely spaced 5{prime} ends and identical 3{prime} ends. The size of each mRNA was about 1.5 kilobases, from both the Northern and S1 data. This size is only slightly longer than that required to code for the 45-kilodalton E{sub 5} protein. In Escherichia coli D318 (celE), the 5{prime} ends of the celE mRNAs are identical to those in T. fusca, but the 3{prime} ends are located ca. 300 base pairs upstream of the T. fusca 3{prime} end. The region where the putative celE promoters were located had some interesting features, including a 60-base-pair A + T-rich sequence and sequence resembling {sigma}{sup 60} promoters.

• OSTI ID: 7165367
• Journal Information: Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 170:9; ISSN JOBAA; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
BACTERIA
CELLULASE
ENZYMES
ESCHERICHIA COLI
GENE REGULATION
GENES
GENETIC MAPPING
GLYCOSYL HYDROLASES
HYBRIDIZATION
HYDROLASES
MAPPING
MESSENGER-RNA
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
RNA
TRANSCRIPTION