Radioprotection of mice with interleukin-1: Relationship to the number of spleen colony-forming units
Compared to saline-injected mice 9 days after 6.5-Gy irradiation, there were twofold more Day 8 spleen colony-forming units (CFU-S) per femur and per spleen from B6D2F1 mice administered a radioprotective dose of human recombinant interleukin-1-alpha (rIL-1) 20 h prior to their irradiation. Studies of this report compared the numbers of CFU-S in nonirradiated mice 20 h after saline or rIL-1 injection. Prior to irradiation, the number of Day 8 CFU-S was not significantly different in the bone marrow or spleens from saline-injected mice and rIL-1-injected mice. Also, in the bone marrow, the number of Day 12 CFU-S was similar for both groups of mice. Similar seeding efficiencies for CFU-S and percentage of CFU-S in S phase of the cell cycle provided further evidence that rIL-1 injection did not increase the number of CFU-S prior to irradiation. In a marrow-repopulation assay, cellularity as well as the number of erythroid colony-forming units, erythroid burst-forming units, and granulocyte-macrophage colony-forming cells per femur of lethally irradiated mice were not increased in recipient mice of donor cells from rIL-1 injected mice. These results demonstrated that a twofold increase in the number of CFU-S at the time of irradiation was not necessary for the earlier recovery of CFU-S observed in mice irradiated with sublethal doses of radiation 20 h after rIL-1 injection.
- Research Organization:
- Armed Forces Radiobiology Research Inst., Bethesda, MD (USA)
- OSTI ID:
- 7018450
- Report Number(s):
- AD-A-214333/7/XAB; AFRRI-SR--89-38
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY
BODY AREAS
BODY FLUIDS
BONE MARROW CELLS
CELL CYCLE
CELL DIVISION
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DRUGS
EFFICIENCY
HEMATOPOIETIC SYSTEM
IRRADIATION
LEGS
LEUKOCYTES
LIMBS
MACROPHAGES
MAMMALS
MATERIALS
MEDICINE
MICE
ORGANS
PHAGOCYTES
PREVENTIVE MEDICINE
RADIATION EFFECTS
RADIATION PROTECTION
RADIOPROTECTIVE SUBSTANCES
RODENTS
SOMATIC CELLS
SPLEEN
SPLEEN COLONY FORMATION
VERTEBRATES