Radioprotection of mice with interleukin-1: Relationship to the number of spleen colony-forming units

Journal Article · Sat Jul 01 04:00:00 EDT 1989 · Radiation Research; (USA)

DOI:https://doi.org/10.2307/3577370· OSTI ID:5669075

Schwartz, G N; Patchen, M L; Neta, R; MacVittie, T J ^[1]

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1. American Red Cross, Rockville, MD (USA)

Compared to saline-injected mice 9 days after 6.5 Gy irradiation, there were twofold more Day 8 spleen colony-forming units (CFU-S) per femur and per spleen from B6D2F1 mice administered a radioprotective dose of human recombinant interleukin-1-alpha (rIL-1) 20 h prior to their irradiation. Studies in the present report compared the numbers of CFU-S in nonirradiated mice 20 h after saline or rIL-1 injection. Prior to irradiation, the number of Day 8 CFU-S was not significantly different in the bone marrow or spleens from saline-injected mice and rIL-1-injected mice. Also, in the bone marrow, the number of Day 12 CFU-S was similar for both groups of mice. Similar seeding efficiencies for CFU-S and percentage of CFU-S in S phase of the cell cycle provided further evidence that rIL-1 injection did not increase the number of CFU-S prior to irradiation. In a marrow repopulation assay, cellularity as well as the number of erythroid colony-forming units, erythroid burst-forming units, and granulocyte-macrophage colony-forming cells per femur of lethally irradiated mice were not increased in recipient mice of donor cells from rIL-1-injected mice. These results demonstrated that a twofold increase in the number of CFU-S at the time of irradiation was not necessary for the earlier recovery of CFU-S observed in mice irradiated with sublethal doses of radiation 20 h after rIL-1 injection.

• OSTI ID: 5669075
• Journal Information: Radiation Research; (USA), Journal Name: Radiation Research; (USA) Vol. 119:1; ISSN 0033-7587; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BODY
BONE MARROW
CELL CYCLE
COLONY FORMATION
GROWTH FACTORS
HEMATOPOIETIC SYSTEM
INJURIES
LYMPHOKINES
MAMMALS
MICE
MITOGENS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIATION EFFECTS
RADIATION INJURIES
RADIATION PROTECTION
RADIOSENSITIVITY EFFECTS
RECOVERY
RODENTS
SPLEEN
TISSUES
VERTEBRATES