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Biological monitoring using cellular macromolecules

Conference · · Preprints of Papers Presented at National Meeting, Division of Water, Air and Waste Chemistry, American Chemical Society; (USA)
OSTI ID:7018216
 [1]
  1. Oak Ridge National Lab., TN (USA)

DNA damage has been proposed as a useful biological marker for assessing the genotoxic properties of environmental pollutants. Many of these pollutants are chemical carcinogens and mutagens with the capacity to cause various types of DNA damage. Genotoxic chemicals may form stable adducts with DNA, adducts which result in the formation of alkali-labile apurinic sites, and unstable adducts which cause strand breaks. Additional damage may occur through the inhibition of normal DNA repair. Each type of damage may contribute toward the eventual transformation of the cell. Analytical techniques that measure DNA damage have been developed at the Oak Ridge National Laboratory and have been used to assess the status of DNA in organisms exposed to chemical carcinogens under controlled laboratory conditions, and to fish and marine mammals from polluted and non-polluted environments. Preliminary data indicate the feasibility of monitoring DNA damage in an organism as a biological marker of environmental genotoxicity.

DOE Contract Number:
AC05-84OR21400
OSTI ID:
7018216
Report Number(s):
CONF-8909236--
Journal Information:
Preprints of Papers Presented at National Meeting, Division of Water, Air and Waste Chemistry, American Chemical Society; (USA), Journal Name: Preprints of Papers Presented at National Meeting, Division of Water, Air and Waste Chemistry, American Chemical Society; (USA) Vol. 28:2; ISSN 0099-7293; ISSN ACWCA
Country of Publication:
United States
Language:
English