DNA damage as a biological marker in aquatic organisms exposed to benzo(a)pyrene

Conference · Wed Dec 31 23:00:00 EST 1986

OSTI ID:5672374

Shugart, L R; Jimenez, B D; McCarthy, J F

We show that minute quantities of BaPDE-DNA adducts in the liver of bluegill sunfish can be detected and quantitated using a simple analytical technique whose sensitivity depends upon the intrinsic fluorescence of the specific adduct being analyzed. These adducts represent damage to DNA of the organism, which, if left uncorrected, could trigger a sequence of events that culminate in the appearance of an overt malignancy. We believe that the data reported here demonstrate that the covalent interaction of genotoxic chemicals with cellular macromolecules such as DNA is, potentially, a sensitive biological marker which could be of early predictive value in assessing exposure and its significance. 13 refs., 1 fig., 1 tab.

• Research Organization: Oak Ridge National Lab., TN (USA)
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 5672374
• Report Number(s): CONF-870940-2; ON: DE88005436
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACUTE EXPOSURE
ADDUCTS
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BENZOPYRENE
CHRONIC EXPOSURE
CONDENSED AROMATICS
DNA ADDUCTS
FISHES
HYDROCARBONS
MUTAGENESIS
ORGANIC COMPOUNDS
RISK ASSESSMENT
VERTEBRATES