Formation and persistence of DNA adducts in liver and kidney of brown bullheads exposed to benzo(a)pyrene (BP)

Conference · Thu Sep 01 00:00:00 EDT 1988 · Preprints of Papers Presented at National Meeting, Division of Water, Air and Waste Chemistry, American Chemical Society; (USA)

OSTI ID:7164548

Sikka, H C; Kandaswami, C; Rutkowski, J P ^[1]; Early, K; Gupta, R C ^[2]

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1. State Univ. of New York College, Buffalo (USA)
2. Baylor College of Medicine, Houston, TX (USA)

Using the {sup 32}P-postlabeling technique, the authors have examined the extent of covalent binding of BP intermediates to DNA of liver and kidney in brown bullheads, the types of DNA adducts formed and the persistence of these adducts. Administration of BP to brown bullheads at a dose of 20 mg/kg (i.p.) resulted in the formation of BP-DNA adducts in both liver and kidney. The formation of the hydrocarbon-DNA adducts in liver proceeded very slowly up to 7 days after treatment. Following this lag, there was a rapid increase in the level of the DNA adducts with the maximum binding noticed 25-30 days after treatment. Subsequently, a substantial decline in the level of BP-DNA adducts occurred. After 42 days of treatment, the total adduct level in liver DNA had decreased to 32% of the maximum adduct level. The general trend for the formation and persistence of BP-DNA adducts in kidney was similar to that noticed with liver except that the maximum DNA adduct formation occurred 12-15 days after treatment and the rate of adduct removal was slower. The predominant adduct ({approximately} 85%) in both liver and kidney DNA was tentatively identified as BP-7,8-dihydrodiol-9,10-epoxide-deoxyguanosine. In addition, four minor DNA adducts, not yet identified, were detected in both organs. On the basis of these data, liver and kidney, a target and non-target organ, respectively, do not seem to differ significantly from each other with respect to the types and magnitude of BP-DNA adducts formed.

• OSTI ID: 7164548
• Report Number(s): CONF-8909236--
• Journal Information: Preprints of Papers Presented at National Meeting, Division of Water, Air and Waste Chemistry, American Chemical Society; (USA), Journal Name: Preprints of Papers Presented at National Meeting, Division of Water, Air and Waste Chemistry, American Chemical Society; (USA) Vol. 28:2; ISSN 0099-7293; ISSN ACWCA
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BENZOPYRENE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
BODY
CARCINOGENESIS
CONDENSED AROMATICS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA ADDUCTS
FISHES
GLANDS
HYDROCARBONS
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LIGHT NUCLEI
LIVER
METABOLISM
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
SENSITIVITY
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES