Formation and persistence of benzo(a)pyrene metabolite-DNA adducts
Benzo(a)pyrene (BP) and other polycyclic aromatic hydrocarbons (PAH) are ubiquitous environmental pollutants and are suspected to be carcinogenic in man. The in vivo formation of BP metabolite-DNA adducts has been characterized in a variety of target and nontarget tissues of mice and rabbits. Tissues included were lung, liver, forestomach, colon, kidney, muscle, and brain. The major adduct identified in each tissue was the (+)-7..beta..,8..cap alpha..-dihydroxy-9..cap alpha..,10..cap alpha..-epoxy-7,8,9,10-tetrahydro-BP (BPDEI)-deoxyguanosine adduct. A 7..beta..,8..cap alpha..-dihydroxy-9..beta..,10..beta..-epoxy-7,8,9,10-tetrahydro-BP (BPDEII)-deoxyguanosine adduct, a (-)-BPDEI-deoxyguanosine adduct, and an unidentified adduct were also observed. The adduct levels are unexpectedly similar in all the tissues examined from the same BP-treated animal. The authors have also examined adduct levels formed in vivo in several cell types of lung and liver. These and previous results strongly suggest that DNA in many human tissues is continuously damaged from known exposure of humans to BP and other PAH. Moreover, DNA adducts formed from BP are persistent in lung and brain. The persistence of adducts in cell types that have slow turnover rates could result in significant accumulation of adducts from long-term exposure to low levels of BP.
- Research Organization:
- National Institute of Environmental Health Sciences, Research Triangle Park, NC
- OSTI ID:
- 5446708
- Journal Information:
- Environ. Health Perspect.; (United States), Journal Name: Environ. Health Perspect.; (United States) Vol. 62; ISSN EVHPA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
ANIMALS
AROMATICS
BENZOPYRENE
BIOCHEMISTRY
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHEMISTRY
CONDENSED AROMATICS
DIGESTIVE SYSTEM
DISTRIBUTION
DNA ADDUCTS
GASTROINTESTINAL TRACT
GLANDS
HYDROCARBONS
INTESTINES
KIDNEYS
LARGE INTESTINE
LIVER
LUNGS
MAMMALS
METABOLITES
MICE
MUSCLES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
RABBITS
RESPIRATORY SYSTEM
RODENTS
STOMACH
TISSUE DISTRIBUTION
VERTEBRATES