Relationship between the rate of hepatic sterol synthesis and the incorporation of (3H)water

Journal Article · Sat Oct 01 04:00:00 EDT 1983 · J. Lipid Res.; (United States)

OSTI ID:7017222

Pullinger, C R; Gibbons, G F

The true rate of sterol synthesis in liver cells was determined by measurement of the weight of desmosterol produced over a given time period during incubations in the presence of triparanol. The simultaneous presence of tritiated water (/sup 3/H/sub 2/O) during the incubations permitted a direct observation of the weight of tritium incorporated into a given mass of newly synthesized sterol. The incorporation of tritium per atom of sterol carbon (H/C ratio) was lower than some previously reported values and suggests that a sizeable proportion of the reducing equivalents (NADPH) required for sterol synthesis arises via the pentose phosphate pathway. The H/C ratio changed significantly with length of the incubation period. The value of the ratio was also dependent upon whether the acetyl-CoA units utilized for sterol synthesis were derived predominantly from a carbohydrate or a fatty acid source.

• Research Organization: Hammersmith Hospital, London, England
• OSTI ID: 7017222
• Journal Information: J. Lipid Res.; (United States), Journal Name: J. Lipid Res.; (United States) Vol. 24:10; ISSN JLPRA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BODY
CHALCOGENIDES
DIGESTIVE SYSTEM
GLANDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIVER
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
RADIONUCLIDE KINETICS
REACTION KINETICS
STEROIDS
STEROLS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TRITIUM OXIDES
UPTAKE