In vivo incorporation of tritium from 3H2O into pulmonary lipids of meal-fed and starved rats

Journal Article · Mon Dec 01 04:00:00 EST 1980 · Am. J. Physiol.; (United States)

OSTI ID:6058954

Todhunter, D A; Scholz, R W

In vivo fatty acid synthesis, as measured by tritium incorporation from 3H2O into fatty acids, was examined in the lungs of meal-fed and starved rats. In meal-fed animals, 74% of the radioactivity isolated from pulmonary lipids was found in the phospholipid fraction. Starving rats for 72 h markedly reduced in vivo 3H2O incorporation into pulmonary lipids. These studies demonstrated net in vivo synthesis of fatty acids in pulmonary tissue of rats using a method that is not complicated by potential differences in metabolic pool sizes or peculiarities of specific carbon substrate sources. Synthesis of fatty acids in vivo was affected by the nutritional state of the animal and citrate appears to be a significant source of cytoplasmic acetyl-CoA for de novo pulmonary lipogenesis in the fed rat.

• OSTI ID: 6058954
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 239:6; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CITRATES
ESTERS
FASTING
HYDROGEN COMPOUNDS
IN VIVO
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
LUNGS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHOSPHOLIPIDS
RATS
RESPIRATORY SYSTEM
RODENTS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
WATER