Cholesterol biosynthesis by the cornea. Comparison of rates of sterol synthesis with accumulation during early development
Journal Article
·
· Journal of Lipid Research; (USA)
OSTI ID:5189202
- Kirksville College of Osteopathic Medicine, MO (USA)
The origin of the cholesterol needed by the cornea for growth and cell turnover was addressed by comparing absolute rates of sterol synthesis with rates of sterol accumulation during early development of the rabbit. Linearity of incorporation of {sup 3}H{sub 2}O and ({sup 14}C)mevalonate into digitonin-precipitable sterols with time of incubation in vitro and a lack of accumulation of {sup 14}C in intermediates of sterol biosynthesis indicated that tritiated water can validly be used to measure rates of sterol synthesis by the cornea. The rate of sterol synthesis per unit weight of rabbit cornea was constant between 14 and 60 days of age at an average 1.03 nmol of {sup 3}H of {sup 3}H{sub 2}O incorporated/mg dry cornea per 8 h. Essentially all of the synthesized cholesterol and most of the cholesterol mass was present in corneal epithelium. The cumulative sterol synthesized over the 46-day period studied exceeded the observed rate of cholesterol accumulation by sixfold. Cholesterol synthesized in excess of the growth requirement was likely used to support turnover of the epithelium which was estimated at 9 days. Removal of cholesterol from the cornea by excretion into tear fluid and clearance by high density lipoproteins are also considered.
- OSTI ID:
- 5189202
- Journal Information:
- Journal of Lipid Research; (USA), Journal Name: Journal of Lipid Research; (USA) Vol. 30:7; ISSN JLPRA; ISSN 0022-2275
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOSYNTHESIS
BODY
BODY AREAS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHOLESTEROL
CLEARANCE
CORNEA
EYES
FACE
HEAD
HYDROGEN COMPOUNDS
HYDROXY ACIDS
HYDROXY COMPOUNDS
IN VITRO
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METABOLISM
MEVALONIC ACID
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
RABBITS
SENSE ORGANS
STEROIDS
STEROLS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
WATER
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOSYNTHESIS
BODY
BODY AREAS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHOLESTEROL
CLEARANCE
CORNEA
EYES
FACE
HEAD
HYDROGEN COMPOUNDS
HYDROXY ACIDS
HYDROXY COMPOUNDS
IN VITRO
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METABOLISM
MEVALONIC ACID
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
RABBITS
SENSE ORGANS
STEROIDS
STEROLS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
WATER