Measurement of daily cholesterol synthesis rates in man by assay of the fractional conversion of mevalonic acid to cholesterol. [/sup 14/C, /sup 3/H]

Journal Article · Fri Jul 01 04:00:00 EDT 1977 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.74.7.3043· OSTI ID:7312215

McNamara, D J; Ahrens, Jr, E H; Samuel, P; Crouse, J R

A significant correlation has been found in man between total daily cholesterol synthesis rates as determined by sterol balance measurements and the fraction of intravenously administered R-(5-/sup 14/C)mevalonic acid converted to cholesterol. In 30 studies it was found that the mean daily cholesterol synthesis rates estimated by sterol balance measurements ranged from 395 to 3047 mg/day, whereas the fractional conversion of mevalonate to cholesterol varied from 0.28 to 0.99. The two parameters correlated with a coefficient of 0.87, P < 0.001. This method for estimating cholesterol synthesis rates requires low doses of radioisotopic materials (25 ..mu..Ci of (/sup 14/C)mevalonate and 5 ..mu..Ci of (/sup 3/H)cholesterol) and less than 1 hr of the patient's time; it can be repeated at intervals of 3 weeks and reflects cholesterol synthesis over a short period of time.

• Research Organization: Rockefeller Univ., New York, NY
• OSTI ID: 7312215
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 74:7; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARBON 14
CARBON ISOTOPES
CARBOXYLIC ACIDS
CHOLESTEROL
EVEN-EVEN NUCLEI
HYDROGEN ISOTOPES
HYDROXY ACIDS
HYDROXY COMPOUNDS
ISOTOPES
LABELLING
LIGHT NUCLEI
MEASURING METHODS
MEVALONIC ACID
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
RADIOASSAY
RADIOISOTOPES
STEROIDS
STEROLS
SYNTHESIS
TRITIUM
YEARS LIVING RADIOISOTOPES