Epidermal growth factor and its receptors in human pancreatic carcinoma

Journal Article · Tue May 01 04:00:00 EDT 1990 · Pancreas; (USA)

DOI:https://doi.org/10.1097/00006676-199005000-00006· OSTI ID:7014919

Chen, Y F; Pan, G Z; Hou, X; Liu, T H; Chen, J; Yanaihara, C; Yanaihara, N ^[1]

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1. Peking Union Medical College Hospital, Beijing (China)

The role of epidermal growth factor (EGF) in oncogenesis and progression of malignant tumors is a subject of vast interest. In this study, radioimmunoassay and radioreceptor assay of EGF were established. EGF contents in malignant and benign pancreatic tumors, in normal pancreas tissue, and in culture media of a human pancreatic carcinoma cell line were determined. EGF receptor binding studies were performed. It was shown that EGF contents in pancreatic carcinomas were significantly higher than those in normal pancreas or benign pancreatic tumors. EGF was also detected in the culture medium of a pancreatic carcinoma cell line. The binding of 125I-EGF to the pancreatic carcinoma cells was time and temperature dependent, reversible, competitive, and specific. Scatchard analysis showed that the dissociation constant of EGF receptor was 2.1 X 10(-9) M, number of binding sites was 1.3 X 10(5) cell. These results indicate that there is an over-expression of EGF/EGF receptors in pancreatic carcinomas, and that an autocrine regulatory mechanism may exist in the growth-promoting effect of EGF on tumor cells.

• OSTI ID: 7014919
• Journal Information: Pancreas; (USA), Journal Name: Pancreas; (USA) Vol. 5:3; ISSN PANCE; ISSN 0885-3177
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOASSAY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
BODY
CARCINOMAS
CELL TRANSFORMATIONS
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
EPIDERMIS
EPITHELIUM
FUNCTIONS
GLANDS
GROWTH FACTORS
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MAN
MEMBRANE PROTEINS
MITOGENS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
RADIORECEPTOR ASSAY
REACTION KINETICS
RECEPTORS
SKIN
TEMPERATURE DEPENDENCE
TIME DEPENDENCE
TISSUES
TRACER TECHNIQUES
VERTEBRATES