Epidermal growth factor binding and receptor distribution in the mouse reproductive tract during development

Journal Article · Thu Nov 01 04:00:00 EST 1990 · Developmental Biology; (USA)

DOI:https://doi.org/10.1016/0012-1606(90)90152-9· OSTI ID:6073223

Bossert, N L; Nelson, K G; Ross, K A; Takahashi, T; McLachlan, J A ^[1]

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1. National Institute of Environmental Health Sciences, Research Triangle Park, NC (USA)

The ontogeny of the epidermal growth factor (EGF) receptor in the different cell types in the neonatal and immature mouse uterus and vagina was examined. Immunohistochemical examination of prenatal and neonatal reproductive tracts with a polyclonal antibody to the EGF receptor shows immunoreactive EGF receptors as early as Day 13 of gestation. Autoradiographic analysis of tissue sections at 3 to 17 days of age (the day of birth is Day 1) demonstrates that both uterine and vaginal epithelial and stromal cells are capable of binding 125I-labeled EGF. Both the 125I-labeled EGF autoradiography and immunohistochemistry in whole tissue show higher EGF receptor levels in the uterine epithelium than the uterine stroma. The presence of EGF receptors was also confirmed by affinity labeling and Scatchard analysis of isolated uterine cell types at 7 and/or 17 days of age. However, in contrast to the autoradiography and immunohistochemistry data of intact tissue, the affinity labeling and Scatchard data of isolated cells indicate that the uterine stroma contains higher levels of EGF receptor than that of the uterine epithelium. The reason for this discrepancy between the different techniques is, as yet, unknown. Regardless of the differences in the actual numbers of EGF receptors obtained, our data demonstrate that the developing mouse reproductive tract contains immunoreactive EGF receptors that are capable of binding 125I-labeled EGF.

• OSTI ID: 6073223
• Journal Information: Developmental Biology; (USA), Journal Name: Developmental Biology; (USA) Vol. 142:1; ISSN 0012-1606; ISSN DEBIA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EPIDERMIS
EPITHELIUM
FEMALE GENITALS
GROWTH FACTORS
IMMUNE REACTIONS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MICE
MITOGENS
NEONATES
NUCLEI
ODD-EVEN NUCLEI
ONTOGENESIS
ORGANIC COMPOUNDS
ORGANS
PREGNANCY
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RODENTS
SKIN
TISSUES
UTERUS
VERTEBRATES