Elevated epidermal growth factor receptor binding in plutonium-induced lung tumors from dogs

Journal Article · Mon Apr 01 04:00:00 EST 1991 · Proceedings of the Society for Experimental Biology and Medicine; (USA)

DOI:https://doi.org/10.3181/00379727-196-43203· OSTI ID:5681062

Leung, F C; Bohn, L R; Dagle, G E ^[1]

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1. Pacific Northwest Lab., Richland, WA (USA)

The objective of this study is to examine and characterize epidermal growth factor receptor (EGF-R) binding in inhaled plutonium-induced canine lung-tumor tissue and to compare it with that in normal canine lung tissue. Crude membrane preparations from normal and lung-tumor tissue from beagle dogs were examined in a radioreceptor assay, using {sup 125}I-labeled epidermal growth factor (EGF) as a ligand. Specific EGF receptor binding was determined in the presence of excess unlabeled EGF. We have examined EGF receptor binding in eight lung-tumor samples obtained from six dogs. Epidermal growth factor receptor binding was significantly greater in lung-tumor samples (31.38%) compared with that in normal lung tissue (3.76%). Scatchard plot analysis from the displacement assay revealed that there was no statistical difference in the binding affinity but significantly higher concentration of EGF-R sites in the lung-tumor tissue (619 fmol/mg) than in normal lung tissue (53 fmol/mg). The increase in EGF-R number in plutonium-induced dog lung tumors does not seem to correlate with increase in the initial lung burden exposure to plutonium. Our results demonstrate that there is a significant increase in EGF-R binding in inhaled plutonium-induced dog lung tumors.

• OSTI ID: 5681062
• Journal Information: Proceedings of the Society for Experimental Biology and Medicine; (USA), Journal Name: Proceedings of the Society for Experimental Biology and Medicine; (USA) Vol. 196:4; ISSN PSEBA; ISSN 0037-9727
• Country of Publication: United States
• Language: English

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560162* -- Radionuclide Effects
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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACTINIDES
ANIMAL TISSUES
ANIMALS
BEAGLES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CARCINOGENESIS
CARCINOMAS
DAYS LIVING RADIOISOTOPES
DISEASES
DOGS
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
EPIDERMIS
EPITHELIUM
EXPERIMENTAL NEOPLASMS
GROWTH FACTORS
IN VITRO
INHALATION
INTAKE
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LUNGS
MAMMALS
MEMBRANE PROTEINS
METALS
MITOGENS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PLUTONIUM
PROTEINS
RADIOINDUCTION
RADIOISOTOPES
RADIORECEPTOR ASSAY
REACTION KINETICS
RECEPTORS
RESPIRATORY SYSTEM
SKIN
TISSUES
TRACER TECHNIQUES
TRANSURANIUM ELEMENTS
VERTEBRATES