Electrogenic symport of glucose and protons in membrane vesicles of Phanerochaete chrysosporium

Journal Article · Sun Jan 01 04:00:00 EST 1984 · Arch. Biochem. Biophys.; (United States)

DOI:https://doi.org/10.1016/0003-9861(84)90050-X· OSTI ID:7014150

Greene, R V; Gould, J M

The white rot fungus, Phanerochaete chrysosporium, is one of the few organisms with documented ability to degrade lignin. Protoplasts from P. chrysosporium were disrupted by osmotic shock and membrane vesicles were isolated from the cell debris. The vesicles exhibit active glucose transport that is consistent with a glucose/H+ symport mechanism. An artificial gradient of H+ (outside greater than inside) stimulates glucose uptake. Conversely, a glucose gradient (outside greater than inside) results in the accumulation of H+ by the vesicles. Glucose uptake is not stimulated by either a Na+ or a K+ gradient. Furthermore, glucose transport is electrogenic, since glucose uptake may be driven by a membrane potential (negative interior) created by K+ diffusion mediated by valinomycin.

• Research Organization: US Dept. of Agriculture, Peoria, IL
• OSTI ID: 7014150
• Journal Information: Arch. Biochem. Biophys.; (United States), Journal Name: Arch. Biochem. Biophys.; (United States) Vol. 228:1; ISSN ABBIA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
ELECTRIC POTENTIAL
FUNGI
GLUCOSE
HEXOSES
MEMBRANE TRANSPORT
MEMBRANES
MONOSACCHARIDES
ORGANIC COMPOUNDS
PHANEROCHAETE
PHYSIOLOGY
PLANTS
SACCHARIDES