Electrogenic sulfate uptake by crustacean hepatopancreatic basolateral membrane vesicles. [Homarus americanus]

Conference · Sun Feb 25 23:00:00 EST 1990 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5489187

Cattey, M A; Gerencser, G A; Aheam, G A ^[1]

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1. Univ. of Hawaii, Honolulu (United States) Univ. of Florida, Gainesville (United States)

Basolateral membrane vesicles (BLMV) were isolated from Atlantic lobster (Homarus americanus) hepatopancreas and purified by discontinuous sucrose gradient centrifugation. BLMV prepared in this fashion were osmotically reactive exhibiting linear dependence of vesicular {sup 35}SO{sub 4}{sup {minus}2} uptake to increasing external osmotic pressure with negligible non-specific isotope binding. Under short circuited conditions (valinomycin/K{sup +}) BLMV responded to either a HCO{sub 3}{sup {minus}} gradient directed out or equilibrated HCO{sub 3}{sup {minus}} (10 mM) by displaying short term accumulation of sulfate above that of equilibrium. Uptake of divalent anion was unaffected by an inwardly directed transmembrane Na{sup +} or tetramethylammonium{sup +} gradient. {sup 35}SO{sub 4}{sup {minus}2}/HCO{sub 3}{sup {minus}} exchange in the presence of valinomycin was stimulated by transient inside positive K{sup +} diffusion potentials and inhibited by transient inside negative K{sup +} diffusion potentials. The role of electrogenic anion exchange by hepatopancreas BLMV in transcellular sulfate transport is discussed.

• OSTI ID: 5489187
• Report Number(s): CONF-9004153--
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 4:3; ISSN FAJOE; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
AMMONIUM COMPOUNDS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
AQUATIC ORGANISMS
ARTHROPODS
BODY
CRUSTACEANS
DECAPODS
DIGESTIVE SYSTEM
DRUGS
ENDOCRINE GLANDS
GLANDS
INVERTEBRATES
ION EXCHANGE
LIVER
LOBSTERS
MEMBRANE TRANSPORT
ORGANS
OXYGEN COMPOUNDS
PANCREAS
PHYSIOLOGY
POTASSIUM COMPOUNDS
SULFATES
SULFUR COMPOUNDS
UPTAKE