Effect of diabetes on in vivo metabolism of (/sup 35/S)-labeled glomerular basement membrane

Journal Article · Sun Jan 01 04:00:00 EST 1984 · Diabetes; (United States)

OSTI ID:7013775

Cohen, M P; Surma, M L

Glomerular basement membrane (GBM) was labeled in vivo by the injection of tracer amounts of (/sup 35/S)-sulfate into normal and streptozotocin-diabetic rats. The biosynthesis and turnover of sulfated glycosaminoglycans in the GBM was determined from the specific activity of (/sup 35/S) after pronase digestion of basement membranes purified from glomeruli isolated 1-7 days after injection. Peak radiolabeling of both normal and diabetic GBM occurred 24 h after injection and, when corrected for differences in serum sulfate specific activities, was less in diabetic than in normal samples. The specific activity of GBM sulfate, expressed as cpm/microgram uronic acid, progressively diminished over the ensuing period of study in both normal and diabetic samples. The rate of decrease in specific activity of (/sup 35/S)-labeled GBM was not significantly different in diabetic preparations compared with that in normal controls. The findings are compatible with diminished sulfation and/or production but normal turnover of glycosaminoglycans in the renal GBM in experimental diabetes.

• Research Organization: Univ. of Medicine and Dentistry, Newark, NJ
• OSTI ID: 7013775
• Journal Information: Diabetes; (United States), Journal Name: Diabetes; (United States) Vol. 33:1; ISSN DIAEA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DIABETES MELLITUS
DISEASES
ENDOCRINE DISEASES
EVEN-ODD NUCLEI
GLOMERULI
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
KINETICS
LABELLING
LIGHT NUCLEI
MAMMALS
MEMBRANES
METABOLIC DISEASES
METABOLISM
MONOCARBOXYLIC ACIDS
MUCOUS MEMBRANES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PATHOLOGICAL CHANGES
PHYSIOLOGY
RADIOISOTOPES
RATS
REACTION KINETICS
RESPONSE MODIFYING FACTORS
RODENTS
SULFATES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
TRACER TECHNIQUES
URONIC ACIDS
VERTEBRATES