/sup 35/S-glycosaminoglycan and /sup 35/S-glycopeptide metabolism by diabetic glomeruli and aorta

Journal Article · Sat May 01 04:00:00 EDT 1982 · Diabetes; (United States)

OSTI ID:5998468

Brown, D M; Klein, D J; Michael, A F; Oegema, T R

/sup 35/S-glycosaminoglycan metabolism by glomeruli isolated from streptozotocin-diabetic and control rats was studied in vivo and in vitro. Total /sup 35/S-glycosaminoglycan synthesis and retention in the matrix by diabetic glomeruli was reduced while degradation was increased. /sup 35/S-glycosaminoglycan content of isolated GBM was similarly decreased. Whereas /sup 35/S-glycosaminoglycan content of glomeruli and GBM was decreased after in vitro incubation with /sup 35/SO/sub 4/, a larger proportion of total /sup 35/S-glycosaminoglycans was found in the incubation medium from diabetic glomeruli. Both control and diabetic glomeruli synthesize /sup 35/S-labeled glycopeptides, the quantity from diabetic glomeruli being reduced. Aorta from /sup 35/SO/sub 4/-injected diabetic rats also synthesized reduced quantities of /sup 35/S-glycosaminoglycans. There were no preferential metabolic alterations of species of /sup 35/S-glycosaminoglycans by diabetic glomeruli or aortas. These studies suggest that synthesis of /sup 35/S-glycosaminoglycans and /sup 35/S-glycopeptides by diabetic glomeruli are altered by disturbances of both synthetic as well as degradative pathways. An alteration of /sup 35/S-glycosaminoglycans interaction with matrix components in diabetes is postulated.

• Research Organization: Department Pediatrics, Laboratory Medicine and Pathology, University of Minnesota, Minneapolis
• OSTI ID: 5998468
• Journal Information: Diabetes; (United States), Journal Name: Diabetes; (United States) Vol. 31; ISSN DIAEA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AORTA
ARTERIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MODELS
BIOLOGICAL PATHWAYS
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DIABETES MELLITUS
DISEASES
ENDOCRINE DISEASES
EVEN-ODD NUCLEI
GLOMERULI
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
METABOLIC DISEASES
METABOLISM
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
VERTEBRATES