((35)S)sulfate incorporation into glomerular basement membrane glycosaminoglycans is decreased in experimental diabetes

Journal Article · Sun Nov 01 04:00:00 EST 1981 · J. Lab. Clin. Med.; (United States)

OSTI ID:5551281

Cohen, M P; Surma, M L

Isolated rat renal glomeruli incorporate radioactive sulfate into glycosaminoglycans, which are integral components of the glomerular basement membrane. Cellulose acetate electrophoresis and specific enzymatic sensitivities of glycosaminoglycans prepared after pronase digestion of purified glomerular basement membrane indicate the presence of heparan sulfate. We examined the effect of experimental diabetes on the incorporation of ((35)S)-sulfate into glycosaminoglycans deposited into newly synthesized glomerular basement membrane in vitro. Basement membranes were purified from glomeruli isolated from normal and streptozotocin-diabetic rats after incubation for 2 hr with radiolabeled sulfate and then were subjected to pronase digestion for isolation of the glycosaminoglycans. ((35)S) incorporation into basement membrane glycosaminoglycans was significantly decreased in glomeruli from diabetic animals. The addition of insulin (100 micron U/ml) in vitro did not affect ((35)S) incorporation into glycosaminoglycans of the glomerular basement membranes in normal or diabetic glomeruli. High glucose concentration (5 vs. 20 mM) was without effect in short-term incubations of glomeruli from normal animals. The results indicate that experimental diabetes influences ((35)S) sulfate incorporation into glomerular basement membrane glycosaminoglycans and suggest that decreased heparan sulfate production and/or sulfation may contribute to the increased permeability of the glomerular basement membrane in diabetes.

• Research Organization: Department of Medicine, Wayne State University School of Medicine, Detroit, MI
• OSTI ID: 5551281
• Journal Information: J. Lab. Clin. Med.; (United States), Journal Name: J. Lab. Clin. Med.; (United States) Vol. 98:5; ISSN JLCMA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
DAYS LIVING RADIOISOTOPES
DIABETES MELLITUS
DISEASES
ENDOCRINE DISEASES
EVEN-ODD NUCLEI
GLOMERULI
GLUCOPROTEINS
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
KINETICS
LIGHT NUCLEI
MAMMALS
MEMBRANES
METABOLIC DISEASES
NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PATHOLOGY
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RODENTS
SACCHARIDES
SULFATES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
TRACER TECHNIQUES
VERTEBRATES