500-MHz proton NMR evidence for two solution structures of the common arm base-paired segment of wheat germ 5S ribosomal RNA

Journal Article · Tue Feb 20 04:00:00 EST 1990 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00459a009· OSTI ID:7008927

Wu, Jiejun; Marshall, A G ^[1]

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1. Ohio State Univ., Columbus (USA)

The base-pair protons of the common arm duplex fragment of wheat germ (Triticum aestivum) ribosomal 5S RNA have been identified and assigned by means of 500-MHz proton NMR spectroscopy. The two previously reported extra base pairs within the fragment are now explained by the presence of two distinct solution structures of the common arm fragment (and its corresponding base-paired segment in intact 5S rRNA). The present conclusions are supported by one- and two-dimensional proton homonuclear Overhauser enhancements in H{sub 2}O and by temperature variation and Mg{sup 2+} titration of the downfield {sup 1}H NMR spectrum. The difference between the two conformers is most likely due to difference in helical tightness. Some additional amino proton resonances have also been assigned.

• OSTI ID: 7008927
• Journal Information: Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 29:7; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALINE EARTH METAL COMPOUNDS
AQUEOUS SOLUTIONS
BARYONS
CATIONS
CEREALS
CHARGED PARTICLES
DISPERSIONS
ELEMENTARY PARTICLES
FERMIONS
GRASS
HADRONS
IONS
LILIOPSIDA
MAGNESIUM COMPOUNDS
MAGNETIC RESONANCE
MAGNOLIOPHYTA
MIXTURES
NUCLEAR MAGNETIC RESONANCE
NUCLEIC ACIDS
NUCLEONS
ORGANIC COMPOUNDS
OVERHAUSER EFFECT
PLANTS
PROTONS
RESONANCE
RIBOSOMAL RNA
RNA
SOLUTIONS
WHEAT