Solution structures of yeast ribosomal 5S and 5. 8S ribonucleic acids via 500-MHz proton nuclear magnetic resonance spectroscopy

Thesis/Dissertation · Wed Jan 01 04:00:00 EST 1986

OSTI ID:6592843

Lee, K M

This investigation reports the first /sup 1/H NMR study of the base-paired secondary structure of yeast 5.8S RNA. Based upon a combination of homonuclear Overhauser enhancements and temperature-dependence of the proton 500 MHz NMR spectrum, eight of the nine base pairs in the most thermally stable helical arm are identified and assigned: G/sub 116/ x C/sub 137/ - C/sub 117/ x G/sub 136/ - C/sub 118/ x G/sub 135/ - C/sub 119/ x G/sub 134/ - C/sub 120/ x G/sub 133/ - U/sub 121/ x G/sub 132/ - U/sub 122/ x A/sub 131/ - G/sub 123/ x C/sub 130/. The present work constitutes the most direct evidence to date for the existence and base-pair sequence of the G x C-rich helix which is common to most currently popular secondary structural models for eukaryotic 5.8S ribosomal RNA. This work also developed a rapid, high-speed, preparative and economical alternative method to Sephadex and high performance size exclusion chromatography for separation of low molecular weight ribosomal RNA's'' 5.8S, 5S, and tRNA.

• Research Organization: Ohio State Univ., Columbus (USA)
• OSTI ID: 6592843
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BARYONS
CONFIGURATION
ELEMENTARY PARTICLES
FERMIONS
FUNGI
HADRONS
HELICAL CONFIGURATION
MAGNETIC RESONANCE
MICROORGANISMS
MOLECULAR STRUCTURE
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEIC ACIDS
NUCLEONS
ORGANIC COMPOUNDS
PLANTS
PROTONS
RESONANCE
RNA
SPECTRA
TEMPERATURE DEPENDENCE
YEASTS