Wheat germ 5S ribosomal RNA common arm fragment conformations observed by sup 1 H and sup 31 P nuclear magnetic resonance spectroscopy
- Ohio State Univ., Columbus (USA)
The nonexchangeable protons of the common arm fragment of wheat germ (Triticum aestivum) ribosomal 5S RNA have been observed by means of high-resolution 500-MHz {sup 1}H NMR spectroscopy in D{sub 2}O solution. Although NMR studies on the exchangeable protons support the presence of two distinct solution structures of the common arm fragment (and of the same base-paired segment in intact 5S rRNA), only a single conformation is manifested in the {sup 1}H NMR behavior of all of the H6 and H5 pyrimidine and most of the H8/H2 purine protons under the same salt conditions. The nonexchangeable protons near the base-paired helix have been assigned by a sequential strategy. Conformational features such as the presence of a cytidine-uridine (C{center dot}U) pair at the loop-helix junction and base stacking into the hairpin loop are evaluated from nuclear Overhauser enhancement spectroscopy (NOESY) data. Double-quantum filtered correlation spectroscopy (DQF-COSY) experiments show that most of the 26 riboses are in the C3{prime}-endo conformation. Finally, backbone conformational changes induced by Mg{sup 2+} and heating have been monitored by {sup 31}P NMR spectroscopy. The results show that the common arm RNA segment can assume two conformations which produce distinguishably different NMR environments at the base-pair hydrogen-bond imino protons but not at nonexchangeable base or ribose proton or backbone phosphate sites.
- OSTI ID:
- 7193002
- Journal Information:
- Biochemistry; (USA), Vol. 29:7; ISSN 0006-2960
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
RIBOSOMAL RNA
NUCLEAR MAGNETIC RESONANCE
AQUEOUS SOLUTIONS
CHEMICAL SHIFT
CONFORMATIONAL CHANGES
CYTIDINE
HEAVY WATER
OVERHAUSER EFFECT
PHOSPHORUS 31
PROTONS
PYRIMIDINES
URIDINE
WHEAT
AZINES
BARYONS
CEREALS
DISPERSIONS
ELEMENTARY PARTICLES
FERMIONS
GRASS
HADRONS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPES
LIGHT NUCLEI
LILIOPSIDA
MAGNETIC RESONANCE
MAGNOLIOPHYTA
MIXTURES
NUCLEI
NUCLEIC ACIDS
NUCLEONS
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS ISOTOPES
PLANTS
RESONANCE
RIBOSIDES
RNA
SOLUTIONS
STABLE ISOTOPES
URACILS
WATER
550601* - Medicine- Unsealed Radionuclides in Diagnostics