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Effects of heavy-atom salts on the luminescence properties of Tetrol I-1 on [alpha]-cyclodextrin/salt matrices

Journal Article · · Applied Spectroscopy; (United States)
;  [1]
  1. Department of Chemistry, University of Wyoming, Laramie, Wyoming 82071 (United States)

Benzo([alpha])pyrene [B(a)P] is one of the most widely studied polyaromatic hydrocarbons. In the human body and in lower animals, B(a)P can become chemically bonded to DNA. Tetrols are the products obtained from the hydrolysis of B(a)P-DNA adducts. It has been proposed that the amount of tetrols detected in human body fluids can be used as a measure of the amount of B(a)P-DNA adducts present in the body. The effects of several heavy-atom salts on the solid-matrix fluorescence and phosphorescence properties of the tetrols were investigated. TINO[sub 3] and TIF were very useful in enhancing the room-temperature phosphorescence (RTP) of the tetrols. For tetrol I-1 on 1:1:8 [alpha]-cyclodextrin:TINO[sub 3]:NaNO[sub 3], the limit of detection was 0.38 femtomole/mg. The RTP approach with the 1:1:8 [alpha]-cyclodextrin:TINO[sub 3]:NaNO[sub 3] matrix would be useful for detecting subfemtomole quantities of the tetrols of B(a)P-DNA adducts from biological samples.

DOE Contract Number:
FG02-86ER13547
OSTI ID:
7008826
Journal Information:
Applied Spectroscopy; (United States), Journal Name: Applied Spectroscopy; (United States) Vol. 48:6; ISSN 0003-7028; ISSN APSPA4
Country of Publication:
United States
Language:
English