Laser-induced room-temperature phosphorescence detection of benzo(a)pyrene-DNA adducts

Journal Article · Wed Apr 15 04:00:00 EDT 1987 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00135a006· OSTI ID:6632458

Vo-Dinh, T; Uziel, M

The room-temperature phosphorescence (RTP) spectrum of benzo(a)pyrene-r-7,t-8,9,10-tetrahydrotetrol (BP-tetrol) has been measured using laser excitation. The BP-terol was obtained by acid hydrolysis of the r-7,t-8-dihydroxy-t-9,10-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene (BPDE)-DNA adducts. BPDE is the ultimate carcinogenic metabolite of benzo(a)pyrene (BP). The BP-tetrol sample was measured on a filter paper substrate pretreated with a heavy-atom salt, thallium acetate, used to increase the phosphorescence signal of BP-tetrol. The detection limit of BPDE in in vitro modified BPDE-DNA was about 15 fmol. The results indicate the RTP would be useful as a simple and practical screening tool for monitoring BPDE-DNA adducts and related BP metabolites in biological samples.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 6632458
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 59:8; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
500200 -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
ADDUCTS
AROMATICS
BENZOPYRENE
CARCINOGENS
CHEMICAL ANALYSIS
CONDENSED AROMATICS
DATA
DNA ADDUCTS
EXPERIMENTAL DATA
HYDROCARBONS
INFORMATION
LASERS
LUMINESCENCE
MEDIUM TEMPERATURE
NUMERICAL DATA
ORGANIC COMPOUNDS
PHOSPHORESCENCE
QUALITATIVE CHEMICAL ANALYSIS