Fluorescence-line-narrowed spectra of polycyclic aromatic carcinogen-DNA adducts

Journal Article · Fri Jan 20 04:00:00 EST 1984 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.6422551· OSTI ID:6153834

Heisig, V; Jeffrey, A M; McGlade, M J; Small, G J

The laser excited fluorescence-line-narrowed spectrum of DNA modified with (+/-)-r-7,t-8-dihydroxy-t-9,10-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene (BPDE), the ultimate carcinogenic metabolite of benzo(a)pyrene (BP), has been obtained in a water-glycerol-ethanol glass at 4.2 K. The spectrum was well resolved and highly characteristic of the chromophore. Comparisons were made between the spectrum of this modified DNA and the isolated deoxyguanosine-BPDE adduct and a series of other 7,8,9,10tetrahydro-BP (THBP) derivatives. 9-Hydroxy-BP 4,5-oxide, which is also involved in the binding of BP to DNA, and THBP have very similar conventional broadband fluorescence spectra. However, the fluorescence-line-narrowed spectra of their derivatives were readily distinguishable either as individual components or as mixtures.

• Research Organization: Columbia Univ., New York, NY
• DOE Contract Number: W-7405-ENG-82
• OSTI ID: 6153834
• Journal Information: Science (Washington, D.C.); (United States), Journal Name: Science (Washington, D.C.); (United States) Vol. 223; ISSN SCIEA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ADDUCTS
AROMATICS
BENZOPYRENE
CONDENSED AROMATICS
DNA ADDUCTS
EMISSION
EMISSION SPECTRA
EMISSION SPECTROSCOPY
ENERGY-LEVEL TRANSITIONS
FLUORESCENCE SPECTROSCOPY
HYDROCARBONS
LASERS
LINE NARROWING
MEASURING METHODS
METABOLIC ACTIVATION
METABOLITES
ORGANIC COMPOUNDS
POLYMERS
SPECTRA
SPECTROSCOPY
STIMULATED EMISSION