Granulocyte-platelet interactions and platelet fibrinogen receptor exposure

Journal Article · Thu Sep 01 00:00:00 EDT 1988 · American Journal of Physiology; (USA)

OSTI ID:7007568

Kornecki, E; Ehrlich, Y H; Egbring, R; Gramse, M; Seitz, R; Eckardt, A; Lukasiewicz, H; Niewiarowski, S ^[1]

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1. Univ. of Vermont, Burlington (USA) University Hospital, Marburg (West Germany) Temple Univ. School of Medicine, Philadelphia, PA (USA)

The authors have examined the interaction of human granulocyte elastase with human platelets. Incubation of human platelets with human granulocyte elastase exposed active fibrinogen-binding sites as evidenced by {sup 125}I-labeled fibrinogen binding and spontaneous fibrinogen-induced platelet aggregation. The aggregation of platelets by fibrinogen occurred at low concentrations of human granulocyte elastase. Platelets pretreated with human granulocyte elastase exposed an average of 10,500 fibrinogen-binding sites per platelet, i.e., about one-third the number of binding sites exposed by optimal concentrations of ADP. With the use of a polyclonal antiplatelet membrane antibody, the glycoproteins IIb (GPIIb), IIIa (GPIIIa), and a 60,000-Da (60 kDa) protein (66 kDa in a reduced system) derived from GPIIIa were immunoprecipitated from the surface of detergent extracts of human {sup 125}I-radiolabeled platelets pretreated with increasing concentrations of human granulocyte elastase. They conclude that (1) the proteolytic action of human granulocyte elastase on platelet GPIIIa results in the formation of two major hydrolytic products, and (2) human granulocyte elastase exposes active fibrongen-binding sites associated with the GPIIb/GPIIIa complex, resulting in direct platelet aggregation by fibrinogen.

• OSTI ID: 7007568
• Journal Information: American Journal of Physiology; (USA), Vol. 255:3; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BLOOD PLATELETS
AUTORADIOGRAPHY
FIBRINOGEN
CROSS-LINKING
RECEPTORS
GLYCOPROTEINS
ENZYMATIC HYDROLYSIS
ELECTROPHORESIS
IODINE 125
LEUKOCYTES
PEPTIDE HYDROLASES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BODY FLUIDS
CHEMICAL REACTIONS
COAGULANTS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
GLOBULINS
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
HYDROLYSIS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
LYSIS
MATERIALS
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
POLYMERIZATION
PROTEINS
RADIOISOTOPES
SOLVOLYSIS
551001* - Physiological Systems- Tracer Techniques