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Loss of fibrinogen receptors from the platelet surface during simulated extracorporeal circulation

Journal Article · · J. Lab. Clin. Med.; (United States)
OSTI ID:5518147
; ; ; ; ;

In vitro recirculation of fresh human heparinized blood in an extracorporeal circuit with a membrane oxygenator decreased fibrinogen- induced platelet aggregation and diminished the number of fibrinogen receptors and glycoprotein IIb/IIIa (GPIIb/GPIIIa) antigenic sites on the platelet surface. In seven experiments, the mean +/- SD Km value for fibrinogen (i.e., molar concentration of fibrinogen required to cause 50% of the maximal rate of aggregation) was 1.58 x 10(-7) mol/L +/- 0.68 x 10(-7) mol/L. After recirculation, this value increased to 3.8 x 10(-7) mol/L +/- 1.94 x 10(-7) mol/L. The maximal aggregation rate of chymotrypsin-treated platelets decreased by 40% after 2 hours of recirculation. The number of fibrinogen receptors on platelets, which were treated with chymotrypsin after a recirculation, decreased from 41,370 +/- 24,000 to 13,230 +/- 10,230/platelet under the same conditions. The number of antigenic sites for monoclonal antibody reacting with GPIIb/GPIIIa complex of adenosine diphosphate-stimulated platelets decreased from 34,200 +/- 5,940 to 19,500 +/- 9,680/platelet after recirculation. Prostaglandin E1 (0. 3 mumol/L) in the perfusion circuit preserved the ability of platelets to react with fibrinogen. In conclusion, the loss of fibrinogen receptors from the surface of platelet membranes results from the interaction of platelets with the surfaces of perfusion circuits.

Research Organization:
Univ. of Pennsylvania Hospital, PA
OSTI ID:
5518147
Journal Information:
J. Lab. Clin. Med.; (United States), Journal Name: J. Lab. Clin. Med.; (United States) Vol. 105:4; ISSN JLCMA
Country of Publication:
United States
Language:
English

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Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADP
AGGLOMERATION
ANIMALS
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD CIRCULATION
BLOOD COAGULATION FACTORS
BLOOD PLATELETS
BODY FLUIDS
CARBOHYDRATES
CHYMOTRYPSIN
COAGULANTS
DRUGS
ENZYMES
FIBRINOGEN
GLOBULINS
GLUCOPROTEINS
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
IN VITRO
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LOSSES
MAMMALS
MAN
MATERIALS
MONOCLONAL ANTIBODIES
NUCLEOTIDES
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PRIMATES
PROSTAGLANDINS
PROTEINS
REACTION KINETICS
RECEPTORS
SACCHARIDES
SERINE PROTEINASES
TRACER TECHNIQUES
VERTEBRATES