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Synthesis by cultured human umbilical vein endothelial cells of two proteins structurally and immunologically related to platelet membrane glycoproteins IIb and IIIa

Journal Article · · J. Cell Biol.; (United States)
DOI:https://doi.org/10.1083/jcb.103.1.81· OSTI ID:5563889
; ; ;

Human platelets participate in a number of adhesive interactions, including binding to exposed subendothelium after vascular injury, and platelet-platelet cohesion to form large aggregates. Platelet membrane glycoproteins (GP) IIb and IIIa constitute a receptor for fibrinogen that, together with fibrinogen and calcium, is largely responsible for mediating the formation of the primary hemostatic plug. Using highly specific polyclonal and monoclonal antibodies as probes, we could detect the presence of both of these glycoproteins in cultured human umbilical vein endothelial cells. Metabolic labeling of endothelium with (/sup 35/S)methionine demonstrated that both GPIIb and GPIIIa were actively synthesized in culture. Using the technique of crossed immunoelectrophoresis, evidence was obtained that the endothelial cell forms of GPIIb and GPIIIa may exist complexed to one another after solubilization in Triton X-100.

Research Organization:
Blood Center of Southeastern Wisconsin, Milwaukee
OSTI ID:
5563889
Journal Information:
J. Cell Biol.; (United States), Journal Name: J. Cell Biol.; (United States) Vol. 103; ISSN JCLBA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD COAGULATION FACTORS
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
COAGULANTS
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
FIBRINOGEN
GLOBULINS
GLUCOPROTEINS
HEMATOLOGIC AGENTS
HEMOSTATICS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MEMBRANES
MONOCLONAL ANTIBODIES
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RADIORECEPTOR ASSAY
SACCHARIDES
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES