Synthesis by cultured human umbilical vein endothelial cells of two proteins structurally and immunologically related to platelet membrane glycoproteins IIb and IIIa
Human platelets participate in a number of adhesive interactions, including binding to exposed subendothelium after vascular injury, and platelet-platelet cohesion to form large aggregates. Platelet membrane glycoproteins (GP) IIb and IIIa constitute a receptor for fibrinogen that, together with fibrinogen and calcium, is largely responsible for mediating the formation of the primary hemostatic plug. Using highly specific polyclonal and monoclonal antibodies as probes, we could detect the presence of both of these glycoproteins in cultured human umbilical vein endothelial cells. Metabolic labeling of endothelium with (/sup 35/S)methionine demonstrated that both GPIIb and GPIIIa were actively synthesized in culture. Using the technique of crossed immunoelectrophoresis, evidence was obtained that the endothelial cell forms of GPIIb and GPIIIa may exist complexed to one another after solubilization in Triton X-100.
- Research Organization:
- Blood Center of Southeastern Wisconsin, Milwaukee
- OSTI ID:
- 5563889
- Journal Information:
- J. Cell Biol.; (United States), Vol. 103
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
FIBRINOGEN
RADIOIMMUNOASSAY
GLUCOPROTEINS
RADIORECEPTOR ASSAY
CELL MEMBRANES
LABELLED COMPOUNDS
MONOCLONAL ANTIBODIES
SULFUR 35
TRACER TECHNIQUES
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD COAGULATION FACTORS
CARBOHYDRATES
CELL CONSTITUENTS
COAGULANTS
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
GLOBULINS
HEMATOLOGIC AGENTS
HEMOSTATICS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MEMBRANES
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
SACCHARIDES
SULFUR ISOTOPES
550201* - Biochemistry- Tracer Techniques