Evaluation of freeze fixation as a phytoplankton preservation method for microautoradiography

Journal Article · Thu Mar 01 00:00:00 EST 1984 · Limnol. Oceanogr.; (United States)

DOI:https://doi.org/10.4319/lo.1984.29.2.0417· OSTI ID:7005874

Paerl, H W

Quantitative microautoradiography of marine and freshwater phytoplankton has been hampered by the fact that chemical techniques used to maintain structural integrity cause leakage of isotopically labeled cell constituents. Chemography, poor preservation of structural integrity, and leakage of cell constituents can all be avoided by quick-freezing filtered samples in liquid N/sub 2/ and then freeze-drying them before autoradiographic preparation. Leakage of fixed /sup 14/C and /sup 33/P and preservation of cell shapes and sizes by these preservation techniques are evaluated in diverse marine and freshwater phytoplankton communities.

• Research Organization: Univ. of North Carolina, Morehead City
• OSTI ID: 7005874
• Journal Information: Limnol. Oceanogr.; (United States), Vol. 29:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PHYTOPLANKTON
AUTORADIOGRAPHY
CYTOLOGICAL TECHNIQUES
CARBON 14
FREEZING
FRESH WATER
PHOSPHATES
PHOSPHORUS 33
SEAWATER
SODIUM CARBONATES
ALKALI METAL COMPOUNDS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBON COMPOUNDS
CARBON ISOTOPES
CARBONATES
DAYS LIVING RADIOISOTOPES
EVEN-EVEN NUCLEI
HYDROGEN COMPOUNDS
ISOTOPES
LIGHT NUCLEI
NUCLEI
ODD-EVEN NUCLEI
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PLANKTON
RADIOISOTOPES
SODIUM COMPOUNDS
WATER
YEARS LIVING RADIOISOTOPES
550701* - Microbiology- Tracer Techniques
550301 - Cytology- Tracer Techniques