Comparisons of three dry application autoradiographic techniques
Conference
·
· Journal of Phycology; (USA)
OSTI ID:5352745
- Virginia Polytechnic Institute and State Univ., Blacksburg (USA)
We compared three common dry application techniques applied for the first time to phytoplankton taking up water-soluble radioisotopically labeled substrates. Following incubation of live phytoplankton communities in oligotrophic, nitrogen-limited Mountain Lake, Virginia, with Carbon-14 labeled methylamine-hydrochloride, an ammomium analog, we concentrated cells on 0.8 um pore size Millipore filters, then preserved filters in liquid nitrogen, and freeze-dried. Differences in the techniques are application of stripping film, preparation of freshly prepared film by dipping loops into liquid emulsion, and dipping cover slips in liquid emulsion. Following dark-incubation and development, autoradiographs were evaluated microscopically for reproducibility, fine resolution of silver grains, background scatter, and eaes of technique.
- OSTI ID:
- 5352745
- Report Number(s):
- CONF-8708345--
- Conference Information:
- Journal Name: Journal of Phycology; (USA) Journal Volume: 23
- Country of Publication:
- United States
- Language:
- English
Similar Records
AUTORADIOGRAPHIC DETECTION OF RADIOACTIVE BACTERIA INTRODUCED INTO SEA WATER AND SEWAGE
LIQUID EMULSION AUTORADIOGRAPHY WITH TRITIUM
New method for labeling and autoradiographic localization of androgen receptors
Journal Article
·
Sun Sep 01 00:00:00 EDT 1963
· Journal of Hygiene (England)
·
OSTI ID:4107882
LIQUID EMULSION AUTORADIOGRAPHY WITH TRITIUM
Journal Article
·
Wed Dec 31 23:00:00 EST 1958
· Laboratory Investigation (U.S.)
·
OSTI ID:4261045
New method for labeling and autoradiographic localization of androgen receptors
Journal Article
·
Wed Jul 01 00:00:00 EDT 1987
· J. Histochem. Cytochem.; (United States)
·
OSTI ID:6332862