Microautoradiography of water-soluble compounds in plant tissue after freeze-drying and pressure infiltration with epoxy resin

Journal Article · Sun Aug 01 04:00:00 EDT 1982 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.70.2.606· OSTI ID:6432978

Vogelmann, T C; Dickson, R E

It is difficult to retain and localize radioactive, water-soluble compounds within plant cells. Existing techniques retain water-soluble compounds with varying rates of efficiency and are limited to processing only a few samples at one time. We developed a modified pressure infiltration technique for the preparation of microautoradiographs of /sup 14/C-labeled, water-soluble compounds in plant tissue. Samples from cottonwood (Populus deltoides Bartr. ex Marsh.) labeled with /sup 14/C were excised, quick frozen in liquid N/sub 2/, freeze-dried at -50/sup 0/C, and pressure-infiltrated with epoxy resin without intermediate solvents or prolonged incubation times. The technique facilitates the mass processing of samples for microautoradiography, gives good cellular retention of labeled water-soluble compounds, and is highly reproducible.

• Research Organization: Forestry Sciences Lab., Rhinelander, WI
• OSTI ID: 6432978
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 70:2; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
CARBON 14 COMPOUNDS
COTTONWOODS
CYTOLOGICAL TECHNIQUES
EPOXIDES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LYOPHILIZATION
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANIC POLYMERS
PETROCHEMICALS
PETROLEUM PRODUCTS
PLANT CELLS
PLANTS
POLYMERS
PRESSURIZING
RESINS
RETENTION
TRACER TECHNIQUES
TREES