In vivo and in vitro dermal penetration of 2,4,5,2 prime ,4 prime , 5 prime -hexachlorobiphenyl in young and adult rats

Journal Article · Sun Oct 01 04:00:00 EDT 1989 · Environmental Research; (USA)

OSTI ID:7004372

Shah, P V; Sumler, M R ^[1]; Fisher, H L; Hall, L L ^[2]

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1. NSI Technology Service Corp., Research Triangle Park, NC (USA)
2. Environmental Protection Agency, Research Triangle Park, NC (USA)

Penetration of 2,4,5,2{prime},4{prime},5{prime}-({sup 14}C)hexachlorobiphenyl (HCB) through skin of young (33 days) and adult (82 days) female Fischer 344 rats was determined in vivo and by two in vitro methods. In vivo dermal penetration at 120 hr was 45% in young and 43% in adults. At 72 hr in vivo dermal penetration was 35% in young and 26% in adults compared to 1.5% for young and 1.0% for adult as measured with a continuous flow in vitro system and 2.9% for young and 1.9% for adults as measured with a static in vitro system. Most of the dermally absorbed HCB remained in the body as only 4.9 and 2.6% of that absorbed was excreted by young and adult rats, respectively, at the end of 120 hr. Significant differences in dermal penetration and kinetics of HCB between young and adult female rats were observed. The elimination of ECB-derived material was approximately six times higher in feces than in urine. A physiological pharmacokinetic model was fitted to the organ and tissue radioactivity distribution data. Parameters in the model determined from dermal dosing of female Fischer 344 rats were in reasonable agreement with those reported in the literature for adult male Sprague-Dawley rats (iv dose). The rate constant for dermal penetration was 0.83 {times} 10{sup {minus}4} min{sup {minus}1} for adults and 0.96 {times} 10{sup {minus}4} min{sup {minus}1} for young. The delay or lag time parameter for dermal penetration was 4.4 hr in adults and 1.1 hr in young.

• OSTI ID: 7004372
• Journal Information: Environmental Research; (USA), Journal Name: Environmental Research; (USA) Vol. 50:1; ISSN ENVRA; ISSN 0013-9351
• Country of Publication: United States
• Language: English

Related Subjects

551001 -- Physiological Systems-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ABSORPTION
ADULTS
AGE GROUPS
ANIMALS
AROMATICS
CHLORINATED AROMATIC HYDROCARBONS
DISTRIBUTION
HALOGENATED AROMATIC HYDROCARBONS
IN VITRO
IN VIVO
ISOTOPE APPLICATIONS
JUVENILES
MAMMALS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
RATS
RODENTS
SKIN ABSORPTION
TISSUE DISTRIBUTION
TRACER TECHNIQUES
UPTAKE
VERTEBRATES