Modulation of microfilament protein composition by transfected cytoskeletal actin genes

Journal Article · Fri Apr 01 04:00:00 EST 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.4.1790· OSTI ID:7000327

Ng, S Y; Erba, H; Latter, G; Kedes, L; Leavitt, J

HuT-14T is a highly tumorigenic fibroblast cell line which exhibits a reduced steady-state level of ..beta..-actin due to coding mutations in one of two ..beta..-actin alleles. The normal rate of total actin synthesis could be restored in some clones of cells following transfection of the functional ..beta..-actin gene but not following transfection of the functional ..gamma..-actin gene. In ..gamma..-actin gene-transfected substrains that have increased rates of ..gamma..-actin synthesis, ..beta..-actin synthesis is further reduced in a manner consistent with an autoregulatory mechanism, resulting in abnormal ratios of actin isoforms. Thus, both ..beta..- and ..gamma..-actin proteins can apparently regulate the synthesis of their coexpressed isoforms. In addition, decreased synthesis of normal ..beta..-actin seems to correlate with a concomitant down-regulation of tropomyosin isoforms.

• Research Organization: Institute of Molecular Biology, Academica Simica, Taipei (TW)
• OSTI ID: 7000327
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:4; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ACTIN
ANIMAL CELLS
BIOSYNTHESIS
CELL CULTURES
CONNECTIVE TISSUE CELLS
CONTROL
FIBROBLASTS
GENES
GENETIC CONTROL
MUTATIONS
ORGANIC COMPOUNDS
PEST CONTROL
PROTEINS
SOMATIC CELLS
SYNTHESIS
TROPOMYOSIN