cap alpha. -skeletal and. cap alpha. -cardiac actin genes are coexpressed in adult human skeletal muscle and heart

Journal Article · Tue Nov 01 04:00:00 EST 1983 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.3.11.1985· OSTI ID:5998725

Gunning, P; Ponte, P; Blau, H; Kedes, L

The authors determined the actin isotypes encoded by 30 actin cDNA clones previously isolated from an adult human muscle cDNA library. Using 3' untranslated region probes, derived from ..cap alpha.. skeletal, ..beta..- and ..gamma..-actin cDNAs and from an ..cap alpha..-cardiac actin genomic clone, they showed that 28 of the cDNAs correspond to ..cap alpha..-skeletal actin transcripts. Unexpectedly, however, the remaining two cDNA clones proved to derive from ..cap alpha..-cardiac actin mRNA. Sequence analysis confirmed that the two skeletal muscle ..cap alpha..-cardiac actin cDNAs are derived from transcripts of the cloned ..cap alpha..-cardiac actin gene. Comparison of total actin mRNA levels in adult skeletal muscle and adult heart revealed that the steady-state levels in skeletal muscle are about twofold greater, per microgram of total cellular RNA, than those in heart. Thus, in skeletal muscle and in heart, both of the sarcomeric actin mRNA isotypes are quite abundant transcripts. They conclude that ..cap alpha..-skeletal and ..cap alpha..-cardiac actin genes are coexpressed as an actin pair in human adult striated muscles. Since the smooth-muscle actins (aortic and stomach) and the cytoplasmic actins (..beta.. and ..gamma..) are known to be coexpressed in smooth muscle and nonmuscle cells, respectively, they postulate that coexpression of actin pairs may be a common feature of mammalian actin gene expression in all tissues.

• Research Organization: The Medigen Project, Dept. of Medicine, and Dept. of Pharmacology, Stanford Medical School, and Veterans Administration Medical Center, Palo Alto, CA 94304
• OSTI ID: 5998725
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 3:11; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ACTIN
ADULTS
AGE GROUPS
ANIMALS
BIOLOGICAL FUNCTIONS
BIOLOGICAL VARIABILITY
BIOSYNTHESIS
BODY
CARDIOVASCULAR SYSTEM
CLASSIFICATION
CLONING
DNA SEQUENCING
DNA-CLONING
FUNCTIONS
GENE REGULATION
GENETIC VARIABILITY
HEART
ISOENZYMES
MAMMALS
MAN
MESSENGER-RNA
MORPHOLOGY
MUSCLES
MYOCARDIUM
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RNA
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
VERTEBRATES