Human cardiac troponin T: Identification of fetal isoforms and assignment of the TNNT2 locus to chromosome 1q
- National Heart and Lung Institute, London (United Kingdom)
- Clare Hall Labs., Potters Bar (United Kingdom)
- Univ. of Maryland, Baltimore, MD (United States)
- Bayer A.G. Institute of Industrial Toxicology, Wuppertal (Germany)
The troponin complex is located on the thin filament of striated muscle and is composed of three component polypeptides: Troponin T, troponin I, and troponin C. Three troponin T genes have been described on the basis of molecular cloning in humans and other vertebrates. These are expressed in a tissue-specific manner and encode the troponin T isoforms expressed in cardiac muscle, slow skeletal muscle, and fast skeletal muscle, respectively. Each of these genes is subject to alternative splicing, resulting in the production of multiple tissue-specific isoforms. The authors have cloned cDNAs encoding human cardiac troponin T from adult heart and have used these to demonstrate that multiple cardiac troponin T mRNAs are present in the human fetal heart, resulting from alternative splicing in the 5[prime] coding region of the gene. Hybridization of the cloned cDNAs to genomic DNA identifies a single-copy gene, and using somatic cell hybrid analysis, the authors have mapped the corresponding gene locus (designated TNNT2) to the long arm of chromosome 1 (1cen-qter). 52 refs., 2 figs., 1 tab.
- OSTI ID:
- 6949957
- Journal Information:
- Genomics; (United States), Journal Name: Genomics; (United States) Vol. 21:2; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
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