Cloning and characterization of a cDNA encoding transformation-sensitive tropomyosin isoform 3 from tumorigenic human fibroblasts

Journal Article · Fri Jan 01 04:00:00 EST 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.1.160· OSTI ID:6925563

Lin, C S; Leavitt, J

The authors isolated a cDNA clone from the tumorigenic human fibroblast cell line HuT-14 that contains the entire protein coding region of tropomyosin isoform 3 (Tm3) and 781 base pairs of 5'- and 3'-untranslated sequences. Tm3, despite its apparent smaller molecular weight than Tm1 in two-dimensional gels, has the same peptide length as Tm1 (284 amino acids) and shares 83% homology with Tm1. Tm3 cDNA hybridized to an abundant mRNA of 1.3 kilobases in fetal muscle and cardiac muscle, suggesting that Tm3 is related to an ..cap alpha../sub fast/-tropomyosin. The first 188 amino acids of Tm3 are identical to those of rat or rabbit skeletal muscle ..cap alpha..-tropomyosin, and the last 71 amino acids differ from those of rat smooth muscle ..cap alpha..-tropomyosin by only 1 residue. Tm3 therefore appears to be encoded by the same gene that encodes the fast skeletal muscle ..cap alpha..-tropomyosin and the smooth muscle ..cap alpha..-tropomyosin via an alternative RNA-splicing mechanism. In contrast to Tm4 and Tm5, Tm3 has a small gene family, with, at best, only one pseudogene.

• Research Organization: Armand Hammer Cancer Research Center, Linus Pauling Institute of Science and Medicine, 440 Page Mill Road, Palo Alto, CA (US)
• OSTI ID: 6925563
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:1; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMAL CELLS
CELL CULTURES
CELL TRANSFORMATIONS
CLEAVAGE
CLONING
CONNECTIVE TISSUE CELLS
CRYSTAL STRUCTURE
DNA-CLONING
ELECTROPHORESIS
FIBROBLASTS
GENE REGULATION
MESSENGER-RNA
MICROSTRUCTURE
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
RNA
RNA PROCESSING
SOMATIC CELLS
TECHNOLOGY ASSESSMENT
TROPOMYOSIN
TWO-DIMENSIONAL CALCULATIONS
TWO-DIMENSIONAL ELECTROPHORESIS