Tropomyosin heterogeneity in human cells

Journal Article · Sun Nov 25 04:00:00 EST 1984 · J. Biol. Chem.; (United States)

OSTI ID:5234482

Giometti, C S; Anderson, N L

Tropomyosin preparations from human platelets, human peripheral blood leukocytes from normal individuals and from a patient with chronic lymphocytic leukemia, human lymphoblastoid cells (GM607), human epithelial cells, and human skin fibroblasts have all been found to contain more than one protein when analyzed by two-dimensional gel electrophoresis. Although the lymphoid cell preparations consistently contain two proteins of almost identical molecular weight (M/sub r/ = 30,000), the platelet, epithelial cell, and fibroblast preparations contain two or more major proteins with molecular weights between 31,000 and 36,000, in addition to a major protein at 30,000. All of these proteins have characteristics in common with tropomyosin including slightly acidic isoelectric point, stability to heat and organic solvents, association with the cytoskeleton, and reactivity with antibody against skeletal muscle tropomyosin. The nonmuscle tropomyosin-like proteins were compared with tropomyosins from human skeletal, cardiac, and smooth muscle by peptide mapping after partial proteolysis. The results showed one of the nonmuscle proteins to be identical to the major smooth muscle tropomyosin in human uterus (myometrium) and another to be similar but not identical to skeletal muscle ..cap alpha..-tropomyosin. The remainder of the proteins with tropomyosin characteristics was unique to nonmuscle cells. In all, nine distinct human proteins with characteristics of tropomyosin are described. Charge variants of two of these proteins have been described previously. 43 references, 7 figures, 2 tables.

• Research Organization: Argonne National Lab., IL
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 5234482
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 259:22; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY
BODY FLUIDS
CHEMISTRY
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROPHORESIS
FIBROBLASTS
HEMIC DISEASES
LEUKEMIA
LEUKOCYTES
MATERIALS
MOLECULAR WEIGHT
NEOPLASMS
ORGANS
PATIENTS
SKIN
SOMATIC CELLS
TROPOMYOSIN
TUMOR CELLS