A variant of human nonmuscle tropomyosin found in fibroblasts by using two-dimensional electrophoresis

Journal Article · Wed Nov 25 04:00:00 EST 1981 · J. Biol. Chem.; (United States)

OSTI ID:5301423

Giometti, C S; Anderson, N L

In an analysis of 12 human fibroblast cell lines by two-dimensional electrophoresis, one cell line (1493) was found to contain a major protein variant (Cytosk:12) not present in any of the other 11 cell lines. Biochemical characterization of the variant protein included determination of its subcellular location, partial amino acid composition, behavior on sodium dodecyl sulfate (SDS) versus SDS/urea gels, and partial proteolytic digestion patterns. All of these methods showed that Cytosk:12 is related to Cytosk:11. Both proteins are located in the cytoskeleton, contain little cysteine or proline and no detectable tryptophan, shift together to a higher apparent molecular weight when electrophoresed in the presence of SDS and 8/sub M/ urea versus SDS alone. Preparation of nonmuscle tropomyosin resulted in the partial purification of both Cytosk:11 and :12. The data suggest that Cytosk:11 is fibroblast nonmuscle tropomyosin and that Cytosk:12 in cell line 1493 is a charge variant of that protein.

• Research Organization: Argonne National Lab., IL
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 5301423
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 256:22; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHEMISTRY
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
EVEN-ODD NUCLEI
FIBROBLASTS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MAN
MOLECULAR WEIGHT
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
RADIOISOTOPES
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
TRITIUM COMPOUNDS
TROPOMYOSIN
VERTEBRATES