Opiate-prostaglandin interactions in the regulation of insulin secretion from rat islets of Langerhans in vitro

Journal Article · Fri Jan 01 04:00:00 EST 1988 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(88)90126-9· OSTI ID:6994955

Green, I C; Tadayyon, M

The inadequate insulin secretory response to glucose stimulation in non-insulin dependent diabetes has been attributed to many factors including high PGE/sub 2/ levels blunting the secretory response, and to the existence of inhibitory opiate activity in vivo. The purpose of the present work was to see if there was a connection between these two independent theories. Radioimmunoassayable PGE/sub 2/ in islets of Langerhans was found to be proportional to islet number and protein content and was typically 4 to 5pg/..mu..g islet protein. Indomethacin sodium salicylate and chlorpropamide all lowered islet PGE/sub 2/ levels and stimulated insulin release in vitro. Dynorphin stimulated insulin release at a concentration of 6 x 10/sup -9/M, while lowering islet PGE/sub 2/. Conversely, at a higher concentration, dynorphin had no stimulatory effect on insulin secretion and did not lower PGE/sub 2/ levels in islets or in the incubation media. The stimulatory effects of dynorphin and sodium salicylate on insulin secretion were blocked by exogenous PGE/sub 2/. PGE/sub 2/ at a lower concentration did not exert any inhibitory effect on dynorphin- or sodium salicylate-induced insulin release. This concentration of exogenous PGE/sub 2/ stimulated insulin release in the presence of 6mM glucose.

• Research Organization: Univ. of Sussex, Brighton (England)
• OSTI ID: 6994955
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 42:21; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BODY
DIABETES MELLITUS
DIGESTIVE SYSTEM
DISEASES
ENDOCRINE DISEASES
ENDOCRINE GLANDS
GLANDS
HORMONES
IMMUNOASSAY
IMMUNOLOGY
IN VITRO
INSULIN
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
METABOLIC DISEASES
ORGANS
PANCREAS
PATHOGENESIS
PEPTIDE HORMONES
PROSTAGLANDINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RATS
REACTION KINETICS
RODENTS
SECRETION
TRACER TECHNIQUES
VERTEBRATES