Phosphatidylcholine biosynthesis and insulin release in rat islets of Langerhans

Thesis/Dissertation · Thu Dec 31 23:00:00 EST 1987

OSTI ID:5413604

Hoffman, J M

Turnover of phosphatidylcholine (PC) has been demonstrated to play a role in glucose stimulation of insulin release by pancreatic islets of Langerhans. The activity of the islet CDP-choline pathway of PC synthesis was determined by measuring the incorporation of radiolabeled choline or {sup 32}PO{sub 4} into PC, phosphorylcholine and CDP-choline. Concurrently, insulin release was measured by radioimmunoassay to correlate insulin release and PC synthesis. Glucose concentrations greater than 8.5 mM stimulated CDP-choline pathway activity. However, measurement of PC lipid phosphorus tended to decrease, suggesting that stimulation of the CDP-choline pathway was a means of replenishing PC pools diminished by hydrolysis of PC. Inhibition of glucose oxidation by mannoheptulose or incubations under hypoxic conditions prevented stimulation of the CDP-choline pathway, while inhibition of phospholipase A{sub 2} (PLA{sub 2}) and secretion by the removal of extracellular Ca{sup 2+} potentiated the stimulation seen with glucose.

• Research Organization: Virginia Commonwealth Univ., Richmond, VA (USA)
• OSTI ID: 5413604
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ALDEHYDES
ALKALINE EARTH METAL COMPOUNDS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
BODY
CALCIUM COMPOUNDS
CARBOHYDRATES
CHOLINE
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM
DRUGS
ENDOCRINE GLANDS
ESTERS
GLANDS
GLUCOSE
HEXOSES
HORMONES
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
INSULIN
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPASE
LIPIDS
LIPOTROPIC FACTORS
MAMMALS
METABOLISM
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PANCREAS
PEPTIDE HORMONES
PHOSPHATES
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
QUATERNARY COMPOUNDS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
RATS
RODENTS
SACCHARIDES
SECRETION
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES