Adenosine kinase deficiency in tritiated deoxyadenosine-resistant mouse S49 lymphoma cell lines

Journal Article · Tue Dec 01 04:00:00 EST 1987 · Biochem. Genet.; (United States)

DOI:https://doi.org/10.1007/BF00502597· OSTI ID:6994914

Sastry, K J; Huang, C; Chan, T S

Mutant sublines were derived of S49 mouse T-lymphoma cells that were resistant to tritiated deoxyadenosine. Twenty-five isolates that were selected in 1 microCi/ml of the nucleoside were cross-resistant to 6-thioguanine, were sensitive to HAT (hypoxanthine, aminopterin, and thymidine), and contained less than 1% of hypoxanthine phosphoribosyltransferase activity in wild-type cells. One of the mutant clones, S49-dA2, was further subjected to selection in a medium containing 2 microCi/ml tritiated deoxyadenosine and 1 microgram/ml deoxycoformycin, an inhibitor of adenosine deaminase. All resistant subclones were cross-resistant to tubercidin, 6-methylmercaptopurine riboside, and arabinosyladenine. One of the subclones, S49-12, was completely devoid of adenosine kinase and was partially deficient in deoxyadenosine kinase. This subclone, however, contained wild-type levels of deoxycytidine kinase. DEAE chromatography of the wild-type cell extracts revealed two deoxyadenosine phosphorylating activities, one of which coeluted with adenosine kinase and was the enzyme missing in S49-12. The other species phosphorylated both deoxyadenosine and deoxycytidine, of which deoxycytidine was the preferred substrate.

• Research Organization: Univ. of Texas Medical Branch, Galveston (USA)
• OSTI ID: 6994914
• Journal Information: Biochem. Genet.; (United States), Journal Name: Biochem. Genet.; (United States) Vol. 25:11-12; ISSN BIGEB
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
ANIMAL CELLS
ANIMALS
CELL DIVISION
CHEMICAL REACTIONS
CHROMATOGRAPHY
DISEASES
ENZYME ACTIVITY
ENZYMES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LYMPHOMAS
MAMMALS
MICE
NEOPLASMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
RIBOSIDES
RODENTS
SENSITIVITY
SEPARATION PROCESSES
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
TUMOR CELLS
VERTEBRATES