6-methylmercaptopurine riboside resistance in human lymphocytes in the in vivo somatic cell mutation test
Journal Article
·
· Environ. Mutagen.; (United States)
Additional drug-resistance markers are being investigated to broaden the in vivo somatic cell mutation test in human lymphocytes (PBL). The adenosine kinase (AK) locus was chosen for study. The adenosine analog 6-methylmercaptopurine riboside (MeMPR) was used as the selective agent for obtaining AK-deficient mutants. Cultures of mitogen-stimulated PBL were set up in the presence (test) and absence (control) of the selective agent. Resistant cells capable of synthesizing DNA in the presence of MeMPR were labeled with tritiated thymidine and enumerated autoradiographically. Human PBL were found to be sensitive to MeMPR inhibition of DNA synthesis and exhibited a dose-dependent decrease in variant frequency (VF) with increasing concentrations of MeMPR. However, no leveling off of the dose-response curve was observed. It was concluded that, because of the autosomal recessive nature of the AK gene, the background Vf in human PBL is too low to allow a useful baseline to be established for the in vivo somatic mutation test.
- Research Organization:
- McMaster Univ., Hamilton, Ontario
- OSTI ID:
- 5507366
- Journal Information:
- Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 8:3; ISSN ENMUD
- Country of Publication:
- United States
- Language:
- English
Similar Records
Diphtheria toxin resistance in human lymphocytes and lymphoblasts in the in vivo somatic cell mutation test
Adenosine kinase deficiency in tritiated deoxyadenosine-resistant mouse S49 lymphoma cell lines
LABELING OF HUMAN TUMOR CELLS IN VIVO BY TRITIATED THYMIDINE
Journal Article
·
Mon Dec 31 23:00:00 EST 1984
· Environ. Mutagen.; (United States)
·
OSTI ID:6181486
Adenosine kinase deficiency in tritiated deoxyadenosine-resistant mouse S49 lymphoma cell lines
Journal Article
·
Mon Nov 30 23:00:00 EST 1987
· Biochem. Genet.; (United States)
·
OSTI ID:6994914
LABELING OF HUMAN TUMOR CELLS IN VIVO BY TRITIATED THYMIDINE
Journal Article
·
Fri Jul 01 00:00:00 EDT 1960
· Laboratory Investigation (U.S.)
·
OSTI ID:4152534
Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
AROMATICS
AUTORADIOGRAPHY
AZAARENES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DRUGS
GENETIC EFFECTS
HETEROCYCLIC COMPOUNDS
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MERCAPTOPURINE
MUTATIONS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PRIMATES
PURINES
PYRIMIDINES
RIBOSIDES
SENSITIVITY
SOMATIC CELLS
SOMATIC MUTATIONS
THIOLS
THYMIDINE
TRITIUM COMPOUNDS
VERTEBRATES
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
AROMATICS
AUTORADIOGRAPHY
AZAARENES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DRUGS
GENETIC EFFECTS
HETEROCYCLIC COMPOUNDS
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MERCAPTOPURINE
MUTATIONS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PRIMATES
PURINES
PYRIMIDINES
RIBOSIDES
SENSITIVITY
SOMATIC CELLS
SOMATIC MUTATIONS
THIOLS
THYMIDINE
TRITIUM COMPOUNDS
VERTEBRATES