Characterization of somatostatin binding to rat anterior pituitary membranes

Conference · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6991728

Galosy, S S; Glenn, K C

Somatostatin binding to its receptor on rat anterior pituitary cell membranes was characterized with (/sup 125/I-Tyr) somatostatin (SRIF). It was found that significant levels of specific /sup 125/I-SRIF binding occurred at 4/sup 0/C following an overnight incubation with pituitary membranes. A parallel amount of specific binding occurred within 10 minutes at 37/sup 0/C. However, specific binding at 37/sup 0/C decreased rapidly over time such that minimal specific binding was observed by 4 hours at 37/sup 0/C. The rapid decline in specific binding over time at 37/sup 0/C suggested that proteases may be degrading the /sup 125/I-SRIF, therefore, the effect of various protease inhibitors was evaluated. No specific binding was observed in the presence of serine protease inhibitors such as PMSF, aprotinin, leupeptin, and soybean trypsin inhibitor. In contrast, the presence of 1 mg/ml of bacitracin, an antibiotic that may be acting as a chelating agent, was essential for specific somatostatin receptor binding. A powerful chelator, 1,10-phenoanthroline, was found to increase the specific binding of /sup 125/I-SRIF, significantly above that achieved in the presence of bacitracin.

• Research Organization: Monsanto Co., St. Louis, MO
• OSTI ID: 6991728
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CELL CONSTITUENTS
CELL MEMBRANES
CHELATING AGENTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
ENZYME INHIBITORS
ENZYMES
GLANDS
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PITUITARY GLAND
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RESPONSE MODIFYING FACTORS
RODENTS
SOMATOSTATIN
TRACER TECHNIQUES
VERTEBRATES