Purification of a putative brain somatostatin receptor

Journal Article · Wed Mar 01 04:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.5.1480· OSTI ID:5085184

He, Haitao; Johnson, K; Thermos, K; Reisine, T ^[1]

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1. Univ. of Pennsylvania School of Medicine, Philadelphia (USA)

The brain somatostatin receptor was purified by affinity chromatographic techniques. A protein of 60 kDa could be purified from rat brain. The protein was eluted from a (D-Trp{sup 8})SRIF affinity column with either sodium acetate (pH 5.5) or free (D-Trp{sup 8})SRIF. The binding of the protein to the affinity column was prevented by free (D-Trp{sup 8})SRIF or the stable SRIF analogue SMS 201-996 but not by the inactive somatostatin 28-(1-14). The purified receptor could be covalently labeled by the {sup 125}I-labeled SRIF analogue CGP 23996. Excess (D-Trp{sup 8})SRIF blocked the binding of {sup 125}I-labeled CGP 23996 to the purified receptor, but somatostatin 28-(1-14) did not affect the binding. A 60-kDa protein was also purified from the anterior pituitary cell line AtT-20, which has a high expression of SRIF receptors. In contrast, no 60-kDa protein could be purified from CHO cells, which have no detectable SRIF receptors. These findings present evidence for the purification of the SRIF receptor.

• OSTI ID: 5085184
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:5; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHO CELLS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MEMBRANE PROTEINS
MOLECULAR STRUCTURE
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHYSIOLOGY
PROTEINS
PURIFICATION
RADIOISOTOPES
RATS
RECEPTORS
RODENTS
SEPARATION PROCESSES
SOMATOSTATIN
VERTEBRATES