High affinity binding of (/sup 125/I-Tyr/sup 11/)somatostatin-14 to human small cell lung carcinoma (NCI-H69)

Journal Article · Fri Jan 01 04:00:00 EST 1988 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(88)90521-8· OSTI ID:6152511

Taylor, J E; Coy, D H; Moreau, J P

The in vitro binding of (/sup 125/I-Try/sup 11/)somatostatin-14 (SRIF-14) to membranes prepared from cultured human small cell lung carcinoma (SCLC) cells (NCI-H69) has been characterized. Binding to SCLC was monophasic and of high affinity. The estimated Bmax was 173 +/- 2.4 fmol/mg protein. Receptors were also present on solid NCI-H69 tumors grown in vivo in the athymic nude mouse. However, the concentration was only about 10% of that observed in cell culture. Biologically-active SRIF analogues were potent inhibitors of (/sup 125/I-Tyr/sup 11/)SRIF-14 binding, and an analysis of the pharmacological specificity indicated that the SCLC receptor was of the peripheral subtype. The presence of SRIF receptors on SCLC membranes may indicate that SRIF has a role in regulation of SCLC function.

• Research Organization: Biomeasure Inc., Hopkinton, MA (USA)
• OSTI ID: 6152511
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 43:5; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CARCINOMAS
CELL CONSTITUENTS
CELL MEMBRANES
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LUNGS
MAMMALS
MAN
MEMBRANE PROTEINS
MEMBRANES
MICE
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RESPIRATORY SYSTEM
RODENTS
SOMATOSTATIN
TRACER TECHNIQUES
TUMOR CELLS
VERTEBRATES