Uric acid provides antioxidant protection against ozone-induced damage for nucleobase, membranes and erythrocytes

Thesis/Dissertation · Wed Jan 01 04:00:00 EST 1986

OSTI ID:6991324

Meadows, J R

The ozone-induced degradation rates of various purine bases, hydroxylated purine compounds, pyrimidine bases, and uric acid were compared. Of the compounds examined, uric acid was the one most readily degraded while the parent compounds, purine and pyrimidine, were the ones most resistant to ozonation. When the breakdown of hydroxylated purines was studied, it was determined that the more OH substituents on the purine, the more readily it was degraded. Because of the preferential attack by ozone on uric acid in solutions containing a nucleic acid base plus uric acid, the presence of the uric acid had a sparing effect on the base. This effect was readily apparent for guanine, thymine, and uracil which were the bases more labile to ozone. Two of the ozonation products of uric acid were identified as allantoin and urea. Ozonation of bovine and swine erythrocyte suspensions resulted in oxidation of oxyhemoglobin to methemoglobin, formation of thiobarbituric acid-reactive materials-a measure of lipid oxidation- and lysis of the red cells. Each of these changes was inhibited by the presence of uric acid in the solution during ozonation.

• Research Organization: Auburn Univ., AL (USA)
• OSTI ID: 6991324
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AROMATICS
AZAARENES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CATTLE
CELL CONSTITUENTS
CELL MEMBRANES
COMPARATIVE EVALUATIONS
DOMESTIC ANIMALS
ERYTHROCYTES
GLOBIN
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
LIPIDS
MAMMALS
MATERIALS
MEMBRANES
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OZONE
PIGMENTS
PORPHYRINS
PROTEINS
PURINES
PYRIMIDINES
RUMINANTS
SWINE
TOXICITY
URIC ACID
VERTEBRATES
XANTHINES